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Evaluation of Enhanced Thermostability and Operational Stability of Carbonic Anhydrase from Micrococcus Species

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Abstract

Carbonic anhydrase (CA) was purified from Micrococcus lylae and Micrococcus luteus with 49.90 and 53.8 % yield, respectively, isolated from calcium carbonate kilns. CA from M. lylae retained 80 % stability in the pH and temperature range of 6.0–8.0 and 35–45 °C, respectively. However, CA from M. luteus was stable in the pH and temperature range of 7.5–10.0 and 35–55 °C, respectively. Cross-linked enzyme aggregates (CLEAs) raised the transition temperature of M. lylae and M. luteus CA up to 67.5 and 74.0 °C, while the operational stability (T 1/2) of CA at 55 °C was calculated to be 7.7 and 12.0 h, respectively. CA from both the strains was found to be monomeric in nature with subunit molecular weight and molecular mass of 29 kDa. Ethoxozolamide was identified as the most potent inhibitor based on both IC50 values and inhibitory constant measurement (K i). The K m and V max for M. lylae CA (2.31 mM; 769.23 μmol/mg/min) and M. luteus CA (2.0 mM; 1,000 μmol/mg/min) were calculated from Lineweaver–Burk plots in terms of esterase activity. Enhanced thermostability of CLEAs alleviates its role in operational stability for application at an on-site scrubber. The characteristic profile of purified CA from Micrococcus spp. advocates its effective application in biomimetic CO2 sequestration.

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References

  1. Lindskog, S. (1997). Pharmacology and Therapeutics, 74, 1–20.

    Article  CAS  Google Scholar 

  2. Zimmerman, S. A., & Ferry, J. G. (2008). Current Pharmacologica Design, 14, 716–721.

    Article  CAS  Google Scholar 

  3. Liu, N., Bond, G. M., Abel, A., McPherson, B., & Stringer, J. (2005). Fuel Processing Technology, 86, 1615–1625.

    Article  CAS  Google Scholar 

  4. Klengel, T., Liang, W. J., Chaloupka, J., Ruoff, C., Schroppel, K., Naglik, J. R., et al. (2005). Current Biology, 15, 2021–2026.

    Article  CAS  Google Scholar 

  5. Bahn, Y. S., Cox, G. M., Perfect, J. R., & Heitman, J. (2005). Current Biology, 15, 2013–2020.

    Article  CAS  Google Scholar 

  6. Elleuche, S., & Poggler, S. (2010). Microbiology, 156, 23–29.

    Article  CAS  Google Scholar 

  7. Sharma, A., & Bhattacharya, A. (2010). Journal of Molecular Catalysis B: Enzymatic, 67, 122–128.

    Article  CAS  Google Scholar 

  8. Sharma, A., Bhattacharya, A., & Shrivastava, A. (2011). Enzyme and Microbial Technology, 48, 416–426.

    Article  CAS  Google Scholar 

  9. Sharma, A., Bhattacharya, A., Pujari, R., & Shrivastava, A. (2008). Indian Journal Microbiology, 48, 365–371.

    Article  CAS  Google Scholar 

  10. Sharma, A., Bhattacharya, A., & Singh, S. (2009). Process Biochemistry, 44, 1293–1297.

    Article  CAS  Google Scholar 

  11. Bond, G. M., Stringer, J., Brandvold, D. K., Simsek, F. A., Medina, N. G., & Egeland, G. (2001). Energy & Fuels, 15, 309–331.

    Article  CAS  Google Scholar 

  12. Gulla, K. C., Gouda, M. D., Thakur, M. S., & Karnath, N. G. (2004). Biosensors and Bioelectronics, 19, 621–625.

    Article  CAS  Google Scholar 

  13. Sheldon, R. A. (2007). Advanced Synthesis and Catalysis, 349, 1289–1307.

    Article  CAS  Google Scholar 

  14. American Public Health Association (APHA) (1985). Standard methods for examination of water and wastewater, 16th ed (pp. 199–200). Washington, DC: American Public health Association.

  15. Kreig, R. N., & Holt, J. G. (1984). Bergey’s manual of systematic bacteriology (Vol. 1). Baltimore: William and Wilkins Co.

    Google Scholar 

  16. Edwards, U., & Rogall, T. H. (1989). Nuclear Acid Research, 17, 7843–7853.

    Article  CAS  Google Scholar 

  17. Pushkas, L. G., Inui, M., Zahan, K., & Yukawa, H. (2000). Microbiology, 46, 2957–2966.

    Google Scholar 

  18. Oritz-Soto, M. E., Rudino-Pinera, E., Rodriguez-Algeria, M. E., & Munguia, A. L. (2009). BMC Biotechnology, 9(68), 1–8.

    Google Scholar 

  19. Schoevaart, R., Wolbers, M. W., Golubovic, M. W., Ottens, M., & Kieboom, A. P. G. (2004). Biotechnology and Bioengineering, 87(6), 754–762.

    Article  CAS  Google Scholar 

  20. Smith, K. S., & Ferry, J. G. (1999). Journal of Bacteriology, 181, 6247–6253.

    CAS  Google Scholar 

  21. Laemmli, U. K. (1970). Nature, 227, 680–685.

    Article  CAS  Google Scholar 

  22. Ekinci, D., Beydemir, S., & Kufreivioglu, O. I. (2007). Journal Enzymology Inhibition and Medicinal Chemistry, 22(6), 745–750.

    Article  CAS  Google Scholar 

  23. Sergeev, V. N., Gerasimenko, L. M., & Zavarzin, G. A. (2002). Mikrobiologiia, 71, 623–637.

    CAS  Google Scholar 

  24. Rawat, M., & Moroney, J. V. (1995). Plant Physiology, 109, 937–944.

    CAS  Google Scholar 

  25. Alber, B. E., & Ferry, J. G. (1994). Proceedings National Academy Science USA, 91, 6909–6913.

    Article  CAS  Google Scholar 

  26. Braus Stromeyer, S. A., Schnappauf, G., Braus, G. H., Gossner, A. S., & Drake, H. L. (1997). Journal of Bacteriology, 179, 7197–7200.

    CAS  Google Scholar 

  27. Ramanan, R., Kannan, K., Vinayagamoorthy, N., Ramkumar, K. M., Sivanesan, S. D., & Chakrabarti, T. (2009). Biotechnology and Bioprocess Engineering, 14, 32–37.

    Article  CAS  Google Scholar 

  28. Smith, K. S., & Ferry, J. G. (2000). FEMS Microbiology Reviews, 24, 335–366.

    Article  CAS  Google Scholar 

  29. Kupriyanova, E., Villarejo, A., Markelova, A., Gerasimenko, L., Zavarzin, G., Samuelsson, G., et al. (2007). Microbiology, 153, 1149–1156.

    Article  CAS  Google Scholar 

  30. Stahler, M. F., Ganter, L., Katherin, L., Manfred, K., & Stephen, B. (2005). FEMS Immunology and Medical Microbiology, 44, 183–189.

    Article  Google Scholar 

  31. Costa, S. A., Tzanov, T., & Carneiro, A. F. (2002). Enzyme Microbial Technology, 30, 387–391.

    Article  CAS  Google Scholar 

  32. Gouda, M. D., Singh, S. A., Appu Rao, A. G., Thakur, M. S., & Karnath, N. G. (2003). Journal of Biological Chemistry, 278(27), 24324–24333.

    Article  CAS  Google Scholar 

  33. Innocenti, A., Muhlschegel, F., Hall, R. A., Steegborn, C., Scozzafava, A., & Supuran, C. T. (2008). Bioorganic & Medicinal Chemistry Letters, 18, 5066–5070.

    Article  CAS  Google Scholar 

  34. Ramanan, R., Kannan, K., Sivanesan, S. D., Mudliar, S., Kaur, S., Tripathi, A. K., et al. (2009). World Journal of Microbiology and Biotechnology, 25, 981–987.

    Article  CAS  Google Scholar 

  35. Armstrong, J. M., Myers, D. A., Verpoorte, J. A., & Edsall, J. T. (1966). Journal of Biological Chemistry, 241(2), 5137–5149.

    CAS  Google Scholar 

  36. Kaur, S., Mishra, M. N., & Tripathi, A. K. (2010). BMC Microbiology, 10, 184–189.

    Article  Google Scholar 

  37. Kaur, S., Mishra, M. M., & Triphathi, A. K. (2009). FEMS Microbiology Letters, 299, 149–158.

    Article  CAS  Google Scholar 

  38. Supuran, C. T. (2008). Current Pharmaceutical Design, 14, 603–614.

    Article  CAS  Google Scholar 

  39. Yadav, R., Satyanaryana, T., Kotwal, S., & Rayalu, S. (2011). Current Science, 100(3), 520–524.

    CAS  Google Scholar 

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Acknowledgments

The authors are thankful to DBT, New Delhi for providing the financial assistance, and A. Bhattacharya is thankful to CSIR New Delhi for granting the CSIR-SRF fellowship.

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  1. Abhishek Bhattacharya

    Present address: Department of Biochemistry, Microbiology and Biotechnology, Rhodes University, Grahamstown, 6140, South Africa

Authors and Affiliations

  1. Bacteriology Laboratory, Department of P.G Studies and Research in Biological Science, Rani Durgavati University, Pachpedi, Jabalpur, 482001, Madhya Pradesh, India

    Abhishek Bhattacharya, Ankita Shrivastava & Anjana Sharma

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  1. Abhishek Bhattacharya
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  2. Ankita Shrivastava
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  3. Anjana Sharma
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Correspondence to Anjana Sharma.

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Bhattacharya, A., Shrivastava, A. & Sharma, A. Evaluation of Enhanced Thermostability and Operational Stability of Carbonic Anhydrase from Micrococcus Species. Appl Biochem Biotechnol 170, 756–773 (2013). https://doi.org/10.1007/s12010-013-0226-y

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  • DOI: https://doi.org/10.1007/s12010-013-0226-y

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