Abstract
Corynebacterium crenatum SYA is an aerobic, Gram-positive, non-sporulating coryneform bacterium, and the mutant C. crenatum strain SYPA 5–5 can produce 30 g/l L-arginine under optimal culture conditions. In this study, the evolution of the cluster argCJBDFRGH (argC~H) involved in arginine biosynthesis in C. crenatum SYA, and SYPA 5–5 was investigated. Compared to the argR of its wild type C. crenatum SYA, a nucleotide substitution (C→T) within the argR gene of the mutant C. crenatum strain SYPA 5–5 was found. The inactivation of ARGR resulted in increased enzyme activities involved in L-arginine biosynthesis and increased L-arginine production in C. crenatum. In contrast, constructing an overexpressing argR C. crenatum/pTR, a complete and functional ARGR decreased the expression of enzymes, depressed transcriptional level of the argC~H cluster, and reduced the production of L-arginine in C. crenatum. It was thus evident that the inactivation of an ARGR suppressor could relieve a bottleneck in downstream steps of the L-arginine biosynthetic pathway, providing a good strategy for improving L-arginine production.
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Acknowledgments
This work was supported by the Program for New Century Excellent Talents in University (NCET-10-0459), the National Basic Research Program of China (973 Program) (2012CB725202), the High-tech Research and Development Programs of China (2012AA022102, 2011AA02A211), the National Natural Science Foundation of China (21276110), the Fundamental Research Funds for the Central Universities (JUSRP51306A, JUSRP1009), and a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
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Xu, M., Rao, Z., Dou, W. et al. The Role of ARGR Repressor Regulation on L-arginine Production in Corynebacterium crenatum . Appl Biochem Biotechnol 170, 587–597 (2013). https://doi.org/10.1007/s12010-013-0212-4
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DOI: https://doi.org/10.1007/s12010-013-0212-4