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Herinase: A Novel Bi-functional Fibrinolytic Protease from the Monkey Head Mushroom, Hericium erinaceum

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Abstract

Herinase, a new bi-functional fibrinolytic metalloprotease, was purified from a medicinal and edible mushroom Hericium erinaceum. The enzyme was monomeric with a molecular mass of 51 kDa. Analysis of fibrin zymography showed an active band with a similar molecular mass. The N-terminal sequence of herinase VPSSFRTTITDAQLRG was highly distinguished from known fibrinolytic enzymes. Moreover, the enzyme activity was strongly inhibited by EDTA and EGTA, indicating that herinase is a metalloprotease. Herinase exhibited high specificity for the substrate t-PA followed by plasmin. The K m and V max values for H-D-Ile-Pro-Arg-PNA were found to be 4.7 mg and 26.7 U/ml respectively. Similarly, fibrin plate assays revealed that it was able to degrade fibrin clot directly and also able to activate plasminogen. Herinase provoked a rapid degradation of fibrin and fibrinogen α chains and slower degradation of γ chains. It had no activity on the β chains of fibrin and fibrinogen. This result suggests that herinase could possibly contain higher amount of α-fibrinogenase. The activity of herinase was stimulated by metal ions such as Ca2+, Mg2+, and Mn2+, but inhibited by Cu2+, Fe2+, and Zn2+. Herinase exhibited maximum activity at 30 °C and pH 7.0. These results demonstrate that herinase could be a novel fibrinolytic enzyme.

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Acknowledgment

This study was supported by research funds from Chosun University (2011).

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Authors and Affiliations

  1. Department of Biotechnology, Chosun University, 375 Seosuk-dong, Dong-gu, Gwangju, 501-759, Republic of Korea

    Bong-Suk Choi, Kumar Sapkota, Jun-Hui Choi & Sung-Jun Kim

  2. Department of Sport and Leisure Studies, Gwangju University, Gwangju, 503-703, Republic of Korea

    Chang-ho Shin

  3. Department of Alternative Medicine, Gwangju University, Gwangju, 503-703, Republic of Korea

    Seung Kim

  4. Jangheung Research Institute for Mushroom Industry, Jangheung, 529-851, Republic of Korea

    Bong-Suk Choi

  5. Central Department of Zoology, Tribhuvan University, Kirtipur, Kathmandu, Nepal

    Kumar Sapkota

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  1. Bong-Suk Choi
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Correspondence to Sung-Jun Kim.

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Choi, BS., Sapkota, K., Choi, JH. et al. Herinase: A Novel Bi-functional Fibrinolytic Protease from the Monkey Head Mushroom, Hericium erinaceum . Appl Biochem Biotechnol 170, 609–622 (2013). https://doi.org/10.1007/s12010-013-0206-2

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