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Sodium Dodecyl Sulphate, a Strong Inducer of Thermostable Glucanhydrolase Secretion from a Derepressed Mutant Strain of Bacillus alcalophilus GCBNA-4

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Abstract

In the present study, we report the optimisation of batch conditions for improved α-1,4-glucan-glucanohydrolase (GGH) secretion by a nitrous acid (NA)-treated Bacillus alcalophilus. The wild (isolate GCB-18) and NA-derivative (mutant GCBNA-4) were grown in a medium containing 10 g/L nutrient broth, 10 g/L starch, 5 g/L lactose, 2 g/L ammonium sulphate, 2 g/L CaCl2 and phosphate buffer (pH 7.6). Sodium dodecyl sulphate (SDS) was used as an enzyme inducer while batch fermentations were carried out at 40 °C. The mutant produced GGH in 40 h which was 15-fold higher than the wild in presence of SDS. Thermodynamic studies revealed that the mutant culture exhibited the capability for improved enzyme activity over a broad range of temperature (35–70 °C). The enzyme was purified by cation-exchange column chromatography with ∼80 % recovery. The performance of fuzzy-logic system control was found to be highly promising for the improved substrate conversion rate. The correlation (1.045E + 0025) among variables demonstrated the model terms as highly significant indicating commercial utility of the culture used (P < 0.05).

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Acknowledgments

Chairman, Department of Botany is thanked for his assistance and moral support. All authors contributed equally in this work. The major part of the work was carried out and completed at Biotechnology Research Centre, Department of Botany. This research received no specific grant from any funding agency in the public, commercial or not for profit sectors.

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Authors and Affiliations

  1. Institute of Industrial Biotechnology (IIB), GC University, Lahore, Pakistan

    Ikram-Ul-Haq

  2. Department of Botany, GC University, Lahore, Pakistan

    Nadia Shamim

  3. H. 30, St. 7, Tezab Ahata, Lahore-39, Pakistan

    Sikander Ali

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  1. Nadia Shamim
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  2. Sikander Ali
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  3. Ikram-Ul-Haq
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Correspondence to Sikander Ali.

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Shamim, N., Ali, S. & Ikram-Ul-Haq Sodium Dodecyl Sulphate, a Strong Inducer of Thermostable Glucanhydrolase Secretion from a Derepressed Mutant Strain of Bacillus alcalophilus GCBNA-4. Appl Biochem Biotechnol 169, 2467–2477 (2013). https://doi.org/10.1007/s12010-013-0139-9

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