Skip to main content

Advertisement

SpringerLink
Log in

Repeated Batch Cell-Immobilized System for the Biotechnological Production of Xylitol as a Renewable Green Sweetener

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The present paper studies the biotechnological production of xylitol using sugarcane bagasse hydrolysate in a repeated batch fermentation system with immobilized cells of Candida guilliermondii FTI20037. Immobilized cell system is considered as an attractive alternative to reuse the well-grown and adapted yeast cells in a new fresh fermentation media, without the need of the inoculum stage. In this work, seven repeated batches were performed in a fluidized bed bioreactor using immobilized cells in calcium alginate beads. According to the obtained results it was observed that the immobilized cells of C. guilliermondii can be reused for six successive batches maintaining an average xylitol yield (Y p/s) of 0.7 g/L and a volumetric productivity (Q p) of 0.42 g/L h at the end of 432 h of fermentation. On the other hand, in the seventh batch (504 h), a decrease of 44 % in the final concentration of xylitol was observed. This reduction can be explained by the possible diffusion and accumulation of insoluble substances, found in the hemicellulosic hydrolysate, in the interior of the immobilization support resulting in substrate mass transfer limitations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Abbreviations

P f :

Final xylitol concentration (in gram xylitol per liter)

P i :

Initial xylitol concentration (in gram xylitol per liter)

Q p :

Overall xylitol production rate (in gram xylitol per liter per hour)

S o :

Initial xylose concentration (in gram xylose per liter)

S f :

Final xylose concentration (in gram xylose per liter)

X rI :

Immobilized cell concentration in the reactor (in gram cells per liter)

Y p/s :

Xylitol yield based on xylose consumption (in gram xylitol per gram xylose)

E :

Conversion efficiency of xylose to xylitol (in percent)

t :

Fermentation time (in hour)

FBR:

Fluidized bed reactor

CV:

The coefficient of variation is defined as the ratio of the standard deviation to the mean

o :

Starting value

p :

Xylitol

s :

Xylose

X :

Biomass

References

  1. Milgrom, P., Ly, K. A., Tut, O. K., Mancl, L., Roberts, M. C., Briand, K., et al. (2009). Xylitol pediatric topical oral syrup to prevent dental caries: a double-blind randomized clinical trial of efficacy. Archives of Pediatrics & Adolescent Medicine, 163(7), 601–607.

    Article  Google Scholar 

  2. Ly, K. A., Milgrom, P., & Rothen, M. (2006). Xylitol, sweeteners, and dental caries. Pediatric Dentistry, 28(2), 154–163.

    Google Scholar 

  3. Uhari, M., Kontiokari, T., Koskela, M., & Niemelä, M. (1996). Xylitol chewing gum in prevention of acute otitis media: double blind randomised trial. British Medical Journal, 313, 1180–1184.

    Article  CAS  Google Scholar 

  4. Parajó, J. C., Domínguez, H., & Domínguez, J. M. (1998). Biotechnological production of xylitol. Part 3: operation in culture media made from lignocellulose hydrolysates. Bioresource Technology, 66, 25–40.

    Article  Google Scholar 

  5. Santos, J. C., Mussatto, S. I., Converti, A., & Silva, S. S. (2005). Influence of aeration rate and carrier concentration on xylitol production from sugarcane bagasse hydrolysate in immobilized-cell fluidized bed reactor. Process Biochemistry, 40, 113–118.

    Article  CAS  Google Scholar 

  6. Cunha, M. A. A., Rodrigues, R. C. B., Santos, J. C., Converti, A., & da Silva, S. S. (2007). Repeated-batch xylitol bioproduction using yeast cells entrapped in polyvinyl alcohol–hydrogel. Current Microbiology, 54, 91–96.

    Article  CAS  Google Scholar 

  7. Santos, J. C., Mussatto, S. I., Dragone, G., Converti, A., & Silva, S. S. (2005). Evaluation of porous glass and zeolite as cells carriers for xylitol production from sugarcane bagasse hydrolysate. Biochemical Engineering Journal, 23(1), 1–9.

    Article  CAS  Google Scholar 

  8. Carvalho, W., Canilha, L., & Silva, S. S. (2008). Semi-continuous xylose-to-xylitol bioconversion by Ca-alginate entrapped yeast cells in a stirred tank reactor. Bioprocess and Biosystems Engineering, 31, 493–498.

    Article  CAS  Google Scholar 

  9. Carvalho, W., Silva, S. S., Vitolo, M., Felipe, M. G. A., & Mancilha, I. M. (2002). Improvement in xylitol production from sugarcane bagasse hydrolysate achieved by the use of a repeated-batch immobilized cell system. Zeitschrift fuer Naturforschung, 57, 109–112.

    CAS  Google Scholar 

  10. Champagne, C. P., Blahuta, N., Brion, F., & Gagnon, C. (2000). A vortex-bowl disk atomizer system for the production of alginate fermentor. Biotechnology and Bioengineering, 68, 681–688.

    Article  CAS  Google Scholar 

  11. Radmann, E. M., Reinehr, C. O., & Costa, J. A. V. (2007). Optimization of the repeated batch cultivation of microalga Spirulina platensis in open raceway ponds. Aquaculture, 265, 118–126.

    Article  Google Scholar 

  12. Huang, W.-C., Chen, S.-J., & Chen, T.-L. (2008). Production of hyaluronic acid by repeated batch fermentation. Biochemical Engineering Journal, 40, 460–464.

    Article  CAS  Google Scholar 

  13. Mussatto, S. I., Rodrigues, L. R., & Teixeira, J. A. (2009). Beta-fructofuranosidase production by repeated batch fermentation with immobilized Aspergillus japonicus. Journal of Industrial Microbiology and Biotechnology, 36, 923–928.

    Article  CAS  Google Scholar 

  14. Meleigy, S. A., & Khalaf, M. A. (2009). Biosynthesis of gibberellic acid from milk permeate in repeated batch operation by a mutant Fusarium moniliforme cells immobilized on loofa sponge. Bioresource Technology, 100, 374–379.

    Article  CAS  Google Scholar 

  15. Liu, D. W., Zeng, R. J., & Angelidaki, I. (2008). Enrichment and adaptation of extreme-thermophilic (70 °C) hydrogen producing bacteria to organic household solid waste by repeated batch cultivation. International Journal of Hydrogen Energy, 33, 6492–6497.

    Article  CAS  Google Scholar 

  16. Yang, X., Wang, B., Cui, F., & Tan, T. (2005). Production of lipase by repeated batch fermentation immobilized Rhizopus arrhizus. Process Biochemistry, 40, 2095–2103.

    Article  CAS  Google Scholar 

  17. Carvalho, W., Silverio, S. S., Converti, A., Vitolo, M., Felipe, M. G. A., Roberto, I. C., et al. (2002). Use of immobilized Candida yeast cells for xylitol production from sugarcane bagasse hydrolysate: cell immobilization conditions. Applied Biochemistry and Biotechnology, 98–100, 489–496.

    Article  Google Scholar 

  18. Sarrouh, B. F., dos Santos, D. T., & Silva, S. S. (2007). Biotechnological production of xylitol in a three phase fluidized bed bioreactor with immobilized yeast cells in Ca-alginate beads. Biotechnology Journal, 2, 1–5.

    Article  Google Scholar 

  19. Sarrouh, B. F., & Silva, S. S. (2010). Application of response surface methodology for optimization of xylitol production from lignocellulosic hydrolysate in a fluidized bed reactor. Chemical Engineering and Technology, 33(9), 1–8.

    Article  Google Scholar 

  20. Sene, L., Felipe, M. G. A., Vitolo, M., Silva, S. S., & Mancilha, I. M. (1998). Adaptation and reutilization of Candida guilliermondii cells for xylitol production in bagasse hydrolysate. Journal of Basic Microbiology, 38, 61–69.

    Article  CAS  Google Scholar 

  21. Carvalho, W., Silva, S. S., Converti, A., & Vitolo, M. (2002). Metabolic behavior of immobilized Candida guilliermondii cells during batch xylitol production from sugarcane bagasse acid hydrolysate. Biotechnology and Bioengineering, 79(2), 165–169.

    Article  CAS  Google Scholar 

  22. Cunha, M. A., Converti, A., Santos, J. C., Ferreira, S. T., & da Silva, S. S. (2009). PVA-hydrogel entrapped Candida guilliermondii for xylitol production from sugarcane hemicellulose hydrolysate. Applied Biochemistry and Biotechnology, 157(3), 527–537.

    Article  Google Scholar 

  23. Silva, D. D. V., Mancilha, I. M., Silva, S. S., & Felipe, M. G. A. (2007). Improvement of biotechnological xylitol production by glucose during cultive of Candida guilliermondii in sugarcane bagasse hydrolysate. Brazilian Archives of Biology and Technology, 50(2), 207–215.

    Article  Google Scholar 

  24. Lima, L. H. A., Felipe, M. G. A., Vitolo, M., & Torres, F. A. G. (2004). Effect of acetic acid present in bagasse hydrolysate on the activities of xylose reductase and xylitol dehydrogenase in Candida guilliermondii. Applied Microbiology and Biotechnology, 65(6), 734–738.

    Article  CAS  Google Scholar 

  25. Rodrigues, R. C. L. B., Sene, L., Matos, G. S., Robserto, I. C., Pessoa, J. R. A., & Felipe, M. G. A. (2006). Enhanced xylitol production by recycling of Candida guilliermondii cells in sugarcane bagasse hemicellulosic hydrolysate. Current Microbiology, 53, 53–59.

    Article  CAS  Google Scholar 

  26. Felipe, M. G. A., Vieira, D. C., Vitolo, M., Silva, S. S., Roberto, I. C., & Mancilha, I. M. (1995). Effect of acetic acid on xylose fermentation to xylitol by Candida guilliermondii. Journal of Basic Microbiology, 35, 171–177.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors would like to thank FAPESP for its financial support.

Author information

Authors and Affiliations

  1. Biotechnology Department, University of São Paulo/Lorena, Campinho Road s/n., P.O. Box: 12600–970, Lorena, São Paulo, Brazil

    Boutros Sarrouh & Silvio Silvério da Silva

  2. Institute of Biological Sciences, Federal University of Minas Gerais (UFMG-ICB), Belo Horizonte, Minas Gerais, CEP 31270-901, Brazil

    Boutros Sarrouh

Authors
  1. Boutros Sarrouh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Silvio Silvério da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Boutros Sarrouh.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sarrouh, B., da Silva, S.S. Repeated Batch Cell-Immobilized System for the Biotechnological Production of Xylitol as a Renewable Green Sweetener. Appl Biochem Biotechnol 169, 2101–2110 (2013). https://doi.org/10.1007/s12010-013-0127-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-013-0127-0

Keywords

Search

Navigation