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Extraction and Characterization of Extracellular Polymeric Substances in Biofilm and Sludge via Completely Autotrophic Nitrogen Removal Over Nitrite System

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Abstract

Extracellular polymeric substances (EPS) were extracted from sludge and biofilm via the completely autotrophic nitrogen removal over nitrite (CANON) system. Tightly bound (TB)-EPS were extracted using four physical methods, namely, cationic exchange resin (CER), sonication, heating, and steaming. CER was the most effective and most suitable method for extraction among the four methods. Moreover, the ultraviolet–vis spectra of TB-EPS indicated that few cells were destroyed by the CER method. The major component contents of total EPS, proteins, carbohydrates, humic substances, and DNA in sludge were 60.77, 49.84, 21.63, and 9.01 mg/g volatile suspended solids (VSS) and 90.03, 29.01, 15.96, and 10.04 mg/g VSS in biofilm, respectively. The Fourier transform infrared (FT-IR) spectra results indicated differences in the EPS functional groups between biofilm and sludge. The results of the batch experiments showed that the biofilm activity was significantly higher than that of the sludge in the CANON system. Furthermore, biomass activity was probably influenced by the EPS composition and distribution in the sludge and biofilm.

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Acknowledgment

We would like to thank the Fundamental Research Funds for the Central Universities (CDJZR11210002) for the financial support.

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Authors and Affiliations

  1. Key Laboratory of the Three Gorges Reservoir Region’s Eco-Environment of MOE, Chongqing University, Chongqing, 400045, China

    You-Peng Chen, Chun Li, Jin-Song Guo, Fang Fang, Xu Gao, Peng Zhang & Shan Li

  2. College of Urban Construction and Environmental Engineering, Chongqing University, Chongqing, 400045, China

    You-Peng Chen, Chun Li, Jin-Song Guo, Fang Fang, Xu Gao, Peng Zhang & Shan Li

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  1. You-Peng Chen
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  2. Chun Li
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  4. Fang Fang
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  6. Peng Zhang
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Correspondence to You-Peng Chen.

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Chen, YP., Li, C., Guo, JS. et al. Extraction and Characterization of Extracellular Polymeric Substances in Biofilm and Sludge via Completely Autotrophic Nitrogen Removal Over Nitrite System. Appl Biochem Biotechnol 169, 526–538 (2013). https://doi.org/10.1007/s12010-012-9996-x

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  • DOI: https://doi.org/10.1007/s12010-012-9996-x

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