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DNA Degradation of Genetically Modified Cottonseed Meal During Feed Processing

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Abstract

The effects of dry heating, wet heating, and extrusion on the degradation of DNA in cottonseed meal (CSM) were studied using polymerase chain reaction (PCR) and real-time PCR approach. Both the sad1 DNA, ranging between 128 and 883 bp in size, and the cry1Ab/c gene, ranging between 183 and 652 bp in size, were detectable in all dry-heated CSM and cottonseed. During wet heating, the sad1 gene (≥883 bp) and the cry1Ab/c (≥952 bp) gene were thoroughly degraded at 105 and 120 °C, respectively. Sizes from 128 to 530 bp for the sad1 gene and sizes from 183 to 652 bp for the cry1Ab/c gene were detected during extrusion at temperatures ranging from 75 to 135 °C. Fragments ≤883 bp for the sad1 gene and ≤952 bp for the cry1Ab/c gene were detected in all of the extruded samples with water content varying between 26 and 34 %. The copy number ratio of cry1Ab/c to sad1 in samples of Bt cottonseed meal decreased rapidly when the temperature increased during the heating process. In conclusion, feed processing markedly degrades the larger DNA fragments of sad1 and cry1Ab/c, with high temperature and water content being the main factors for that degradation.

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Acknowledgments

This study was supported by the National Major Project of Breeding for Genetically Modified Organism in China (no. 2009ZX 08012-012B) and the National Natural Science Foundation of China (no. 31201828).

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Authors and Affiliations

  1. Key Laboratory of Feed Biotechnology, Ministry of Agriculture, Beijing, 100081, People’s Republic of China

    Qingfeng Guan, Xiumin Wang, Da Teng, Yalin Yang & Jianhua Wang

  2. Gene Engineering Laboratory, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun Nandajie St., Haidian District, Beijing, 100081, People’s Republic of China

    Qingfeng Guan, Xiumin Wang, Da Teng, Yalin Yang & Jianhua Wang

Authors
  1. Qingfeng Guan
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  2. Xiumin Wang
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  3. Da Teng
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  5. Jianhua Wang
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Correspondence to Jianhua Wang.

Additional information

Qingfeng Guan and Xiumin Wang contributed equally into this paper.

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Guan, Q., Wang, X., Teng, D. et al. DNA Degradation of Genetically Modified Cottonseed Meal During Feed Processing. Appl Biochem Biotechnol 169, 368–379 (2013). https://doi.org/10.1007/s12010-012-9982-3

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  • DOI: https://doi.org/10.1007/s12010-012-9982-3

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