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Genotypic Variability Among Soybean Genotypes Under NaCl Stress and Proteome Analysis of Salt-Tolerant Genotype

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Abstract

The present investigation was conducted to evaluate salt tolerance in ten genotypes of soybean (Glycine max L.). Twelve-day-old seedlings, grown hydroponically, were treated with 0, 25, 50, 75, 100, 125 and 150 mM NaCl for 10 days. Growth, lipid peroxidation and antioxidant enzyme activities were evaluated. Growth, measured in terms of length, fresh weight and dry weight of plants, was drastically reduced in Pusa-24 while there was little effect of NaCl treatment on Pusa-37 genotype of soybean. High level of lipid peroxidation was observed in Pusa-24 as indicated by increased level of malondialdehyde. Activities of superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase were maximum in Pusa-37 where 9-, 1-, 5- and 6-fold increase over control were observed, respectively. The results suggested that Pusa-24 and Pusa-37 are salt-sensitive and salt-tolerant genotype of soybean, respectively, and antioxidant defence system is involved in conferring the sensitiveness and tolerance in these genotypes. Salt-tolerant genotype Pusa-37, was further analysed by 2-dimensional gel electrophoresis to analyse the differential expression of proteins at high salt stress. In the present study, 173 protein spots were identified. Of these, 40 proteins were responsive to salinity in that they were either up- or downregulated. This study could help us in identifying the possible regulatory switches (gene/s) controlling novel proteins of the salt-tolerant genotype of the crop plants and their possible role in defence mechanism.

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Abbreviations

APX:

Ascorbate peroxidase

BSA:

Bovine serum albumin

CAT:

Catalase

DW:

Dry weight

EDTA:

Ethylenediaminetetraacetic

FW:

Fresh weight

GR:

Glutathione reductase

GSH:

Reduced glutathione

GSSG:

Oxidised glutathione

MDA:

Malondialdehyde

NBT:

Nitroblue tetrazolium

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

TBA:

Thiobarbituric acid

TCA:

Trichloroacetic acid

MALDI-TOF-MS:

Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry

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Author notes

  1. Khalid Rehman Hakeem

    Present address: Faculty of Forestry, Universiti Putra Malaysia (UPM), Serdang, 43400, Selangor, Malaysia

Authors and Affiliations

  1. Department of Botany, Faculty of Science, Jamia Hamdard, New Delhi, 110062, India

    Khalid Rehman Hakeem, Khalid Rehman Hakeem, Ruby Chandna, Tariq Omer Siddiqui & Muhammad Iqbal

  2. Department of Microbiology, King Saud University, Riyadh, Saudi Arabia

    Faheema Khan

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  1. Khalid Rehman Hakeem
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Correspondence to Khalid Rehman Hakeem.

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Khalid Rehman Hakeem, Faheema Khan and Ruby Chandna contributed equally to this work.

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Hakeem, K.R., Khan, F., Chandna, R. et al. Genotypic Variability Among Soybean Genotypes Under NaCl Stress and Proteome Analysis of Salt-Tolerant Genotype. Appl Biochem Biotechnol 168, 2309–2329 (2012). https://doi.org/10.1007/s12010-012-9939-6

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