Abstract
Stable carriers are required for gene delivery. The use of polyethyleneimine (PEI) has been researched extensively; however, it is not suitable for targeted gene delivery to specific cells. To provide a targeting ability of the specific antibody to PEI, two repeats of Fc-binding domain of protein G (C2) were utilized. The constructed protein containing C2 could bind to a specific antibody and form a larger complex with plasmid DNA/PEI complex. The specific antibody to cell surface protein was bound to the complex through the use of C2 domain, and it was added to cells. As the result, the efficient expression of delivered reporter gene could be realized.
Similar content being viewed by others
References
Boussif, O., Lezoualc’h, F., Zanta, M. A., Mergny, M. D., Scherman, D., Demeneix, B., et al. (1995). Proceedings of the National Academy of Sciences of the United States of America, 92, 7297–7301.
Hattori, Y., & Maitani, Y. (2007). Biological & Pharmaceutical Bulletin, 30, 1773–1778.
Wu, H. C., Chang, D. K., & Huang, C. T. (2006). Journal of Cancer Molecules, 2, 57–66.
Strehblow, C., Schuster, M., Moritz, T., Kirch, H. C., Opalka, B., & Petri, J. B. (2005). Journal of Controlled Release, 102, 737–747.
Han, J. Y., Choi, D. S., Kim, C., Joo, H., & Min, C. K. (2008). Cancer Biotherapy and Radiopharmaceutical, 23, 247–258.
Suzuki, M., Takayanagi, A., & Shimizu, N. (2004). Cancer Science, 95, 424–429.
Akerstrom, B., Brodin, T., Reis, K., & Bjorck, L. (1985). Journal of Immunology, 135, 2589–2592.
Kobatake, E., Yamano, R., & Mie, M. (2011). Applied Biochemistry and Biotechnology, 163, 296–303.
Tanaka, G., Funabashi, H., Mie, M., & Kobatake, E. (2006). Analytical Biochemistry, 350, 298–303.
Zhang, C., Yadava, P., & Hughes, J. (2004). Methods, 33, 144–150.
Godbey, W. T., Wu, K. K., & Mikos, A. G. (1999). Journal of Biomedical Materials Research, 45, 268–275.
Godbey, W. T., Wu, K. K., & Mikos, A. G. (2001). Biomaterials, 22, 471–480.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Bae, J., Mie, M. & Kobatake, E. Targeted Gene Delivery via PEI Complexed with an Antibody. Appl Biochem Biotechnol 168, 2184–2190 (2012). https://doi.org/10.1007/s12010-012-9928-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-012-9928-9