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Elicitation of Andrographolide in the Suspension Cultures of Andrographis paniculata

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Abstract

Andrographis paniculata belonging to the family Acanthaceae produces a group of diterpene lactones, one of which is the pharmaceutically important—andrographolide. It is known to possess various important biological properties like anticancer, anti-HIV, anti-inflammatory, etc. This is the first report on the production of andrographolide in the cell suspension cultures of Andrographis paniculata by ‘elicitation’. Elicitation was attempted to enhance the andrographolide content in the suspension cultures of Andrographis paniculata and also to ascertain its stimulation under stress conditions or in response to pathogen attack. The maximum andrographolide production was found to be 1.53 mg/g dry cell weight (DCW) at the end of stationary phase during the growth curve. The biotic elicitors (yeast, Escherichia coli, Bacillus subtilis, Agrobacterium rhizogenes 532 and Agrobacterium tumefaciens C 58) were more effective in eliciting the response when compared to the abiotic elicitors (CdCl2, AgNO3, CuCl2 and HgCl2). Yeast has shown to stimulate maximum accumulation of 13.5 mg/g DCW andrographolide, which was found to be 8.82-fold higher than the untreated cultures.

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References

  1. Koehn, F. E., & Carter, G. T. (2005). Nature Reviews. Drug Discovery, 4, 206–220.

    Article  CAS  Google Scholar 

  2. Leonard, E., Runguphan, W., Connor, & Prather, K. J. (2009). Nature Chemical Biology, 5, 292–300.

    Article  CAS  Google Scholar 

  3. Sheeja, K., Shihab, P. K., & Kuttan, G. (2006). Immunopharmacology and Immunotoxicology, 28, 129–140.

    Article  CAS  Google Scholar 

  4. Shen, Y. C., Chen, C. F., & Chiou, W. (2002). British Journal of Pharmacology, 135, 399–406.

    Article  CAS  Google Scholar 

  5. Wiart, C., Kumar, K., Yusof, M. Y., Hamimah, H., Fauzi, Z. M., & Sulaiman, M. (2005). Phytotherapy Research, 19, 1069–1070.

    Article  CAS  Google Scholar 

  6. Handa, S., & Sharma, A. (1990). Indian Journal of Medical Research, 92, 284–292.

    CAS  Google Scholar 

  7. Chander, R., Srivastava, V., Tandon, J. S., & Kapoor, N. K. (1995). Pharmaceutical Biology, 33, 135–138.

    Article  CAS  Google Scholar 

  8. Trivedi, N., & Rawal, U. M. (2000). Indian Journal of Pharmacology, 32, 288–293.

    Google Scholar 

  9. Visen, P. K. S., Saraswat, B., Vuksen, V., Dhawan, B. N.(2007). Journal of Complement and Integrated Medicine, 4–10.

  10. Tan, B. K. H., & Zhang, C. Y. (2004). Andrographis paniculata and the cardiovascular system. In L. Packer, C. N. Ong, & B. Halliwell (Eds.), Herbal and traditional medicine: Molecular aspects on health (pp. 441–456). Taipei: CRC.

    Google Scholar 

  11. Kumar, R. A., Sridevi, K., Kumar, N. V., Nanduri, S., & Rajagopal, S. (2004). Journal of Ethnopharmacology, 92, 291–295.

    Article  CAS  Google Scholar 

  12. Rajagopal, S., Kumar, R. A., Deevi, D. S., Satyanarayana, C., & Rajagopal, R. (2003). Journal of Experimental Therapeutics and Onclogy, 3, 147–158.

    Article  CAS  Google Scholar 

  13. Cheung, H. Y., Cheung, S. H., Li, L., Cheung, C. S., Lai, W. P., Fong, W. F., & Leung, F. M. (2005). Planta Medica, 71, 1106–1111.

    Article  CAS  Google Scholar 

  14. Li, J., Cheung, H. Y., Zhang, Z., Chan, G. K. L., & Fong, W. F. (2007). European Journal of Pharmacology, 3, 31–44.

    Article  Google Scholar 

  15. Sukardiman, H., Widyawaruyanti, A., Sismindari, Zaini, N. C. (2007). African Journal of Traditional Complement and Alternative Medicines, 4, 345–351

  16. Zhou, J., Zhang, S., Ong, C., & Shen, H. (2006). Biochemical Pharmacology, 72, 132–144.

    Article  CAS  Google Scholar 

  17. Sharma, A., Singh, R. T., Sehgal, V., & Handa, S. S. (1991). Fitoterapia, 62, 131–138.

    Google Scholar 

  18. Tang, W., Eisenbrand, G., Chinese Drugs of Plant Origin. (1992). Chemistry, pharmacology, and use in traditional and modern medicine (pp. 97–103). Berlin: Springer

  19. Calabrese, C., Berman, S. H., Babish, J. G., Ma, X., Shinto, L., Dorr, M., Wells, K., Wenner, C. A., & Standish, L. J. (2000). Phytotherapy Research, 14, 333–338.

    Article  CAS  Google Scholar 

  20. Otake, T., Mori, H., Morimoto, M., Ueba, N., Sutardjo, S., Kusumoto, I. T., Hattori, M., & Namba, T. (1995). Phytotherapy Research, 9, 6–10.

    Article  Google Scholar 

  21. Iruetagoyena, M. I., Tobar, J. A., Gonzalez, P. A., Sepulveda, S. E., Figueroa, C. A., Burgos, R. A., Hancke, J. L., & Kalergis, A. M. (2005). Journal of Pharmacology and Experimental Therapeutics, 5, 366–372.

    Google Scholar 

  22. Dornenburg, H., & Knorr, D. (1995). Enzyme and Microbial Technology, 17, 674–684.

    Article  Google Scholar 

  23. Rao, S. R., & Ravishankar, K. A. (2002). Biotechnology Advances, 20, 101–153.

    Article  CAS  Google Scholar 

  24. Luczkiewicz, M. (2008). Research into isoflavonoids: phytoestrogens in plant cell cultures. In K. G. Ramawat & J. M. Merillon (Eds.), Bioactive molecules and medicinal plants (pp. 54–84). Berlin: Springer.

    Google Scholar 

  25. Vijai, S. K., Rinki, J., Priti, T., & Dixit, V. K. (2010). In Vitro Cell and Developmental Biology—Plant, 46, 354–362.

    Article  Google Scholar 

  26. Masoumeh, K., Tahereh, H., & Sayyed, K. K. T. (2010). Plant Omics Journal, 3(4), 109–114.

    Google Scholar 

  27. Dass, S., & Ramawat, K. G. (2009). Plant Cell Tissue Organ culture, 96, 349–353.

    Article  Google Scholar 

  28. Krolicka, A., Kartanowicz, R., Wosinskia, S., Zpitter, A., Kaminski, M., & Lojkowska, E. (2006). Enzyme and Microbial Technology, 39, 1386–1389.

    Article  CAS  Google Scholar 

  29. Kim, H. J., Chen, F., Wang, X., & Rajapakse, N. C. (2005). Journal of Agricultural and Food Chemistry, 53(9), 3696–3701.

    Article  CAS  Google Scholar 

  30. Broeckling, C. D., Huhman, D. V., Farag, M. A., Smith, J. T., May, G. D., Mendes, P., Dixon, R. A., & Sumner, L. W. (2005). Journal of Experimental Botany, 56(410), 323–336.

    Article  CAS  Google Scholar 

  31. Wyk, V. B., & Wink, M. (2004). Medicinal plants of the world: an illustrated scientific guide to important medicinal plants and their uses (p. 480). Portland: Timber.

    Google Scholar 

  32. Kim, O. T., Kim, M. Y., Hong, M. H., Ahn, J. C., & Hwang, B. (2004). Plant Cell Reports, 23, 339–344.

    Article  CAS  Google Scholar 

  33. Namdeo, A. G. (2007). Pharmacognosy Reviews, 1(1), 69–79.

    CAS  Google Scholar 

  34. Komaraiah, P., Naga, A. R., Kavi Kishor, P. B., & Ramakrishna, S. V. (2002). Enzyme and Microbial Technology, 31(5), 634–639.

    Article  CAS  Google Scholar 

  35. Ashish, B., Singh, D., & Vinod, D. K. (2008). Applied Biochemistry and Biotechnology, 151, 556–564.

    Article  Google Scholar 

  36. Ajungla, L., Patil, P. P., Barmukh, R. B., & Nikam, T. D. (2009). Indian Journal of Biotechnology, 8, 317–322.

    CAS  Google Scholar 

  37. Bhuvaneswari, C., Kiranmayee, R., Suryakala, G., & Archana, G. (2012). World Journal of Microbiology and Biotechnology, 28, 741–747.

    Google Scholar 

  38. Beena, M. R., & Martin, K. P. (2003). In Vitro Cellular & Developmental Biology – Plant, 39(5), 510–513.

    Article  Google Scholar 

  39. Maridass, M., Mahesh, R., Raju, G., & Muthuchelian, K. (2010). International Journal of Bological Technology, 1(1), 33–37.

    CAS  Google Scholar 

  40. Sharma, A., Krishan, L., & Handa, S. S. (1952). Phytochemical Analysis, 3, 129–131.

    Article  Google Scholar 

  41. Praveen, N., Manohar, S. H., Naik, P. M., Nayeem, A., Jeong, J. H., & Murthy, H. N. (2009). Current Science, 96(5), 694–697.

    CAS  Google Scholar 

  42. Shilpa, K., Varun, K., & Lakshmi, B. S. (2010). Journal of Plant Science, 5(3), 222–247.

    Article  Google Scholar 

  43. Fatima, B., Muhammad, A., & Seema, I. (2008). Asian journal of Scientific Research, 2, 160–165.

    Google Scholar 

  44. Zhang, C. H., Wu, J. Y., & He, G. Y. (2002). Applied Microbiology and Biotechnology, 60, 396–402.

    Article  CAS  Google Scholar 

  45. Enrique, O., MartoAnez-Solano, J. R., Piqueras, A., & Hello, E. (2003). Journal of Experimental Botany, 54, 291–301.

    Article  Google Scholar 

  46. Zhao, J., Davis, L. C., & Verpoorte, R. (2005). Biotechnology Advances, 23, 283–333.

    Article  CAS  Google Scholar 

  47. Abu-Jawdah, Y. (1982). Phytopathology, 103, 272–279.

    Article  CAS  Google Scholar 

  48. De Tapia, M., Berbman, P., Awade, A., & Burkard, G. (1986). Plant Sciences, 45, 167–177.

    Article  Google Scholar 

  49. Nasser, W., De Tapia, M., Kauffmann, S., Kouhsari, M. S., & Burkard. (1988). Plant Molecular Biology, 1(1), 529–538.

    Article  Google Scholar 

  50. Ham, K. S., Kauffmann, S., Albersheim, P., & Darvill, A. G. (1991). Host pathogen Interactions XXXIX. A soybean pathogenesis-related protein with b-1, 3-glucanase activity releases phytoalexin elicitor-active heat stable fragments from fungal walls. Molecular Plant-Microbe Interactions, 4, 545–552.

    Article  CAS  Google Scholar 

  51. Jiang, L. Z., Zhou, L. G., & Wu, J. Y. (2010). Applied Microbiology and Biotechnology, 87, 137–144.

    Article  Google Scholar 

  52. Lary, J. H., & Gary, S. (1986). Microbiology reviews, 50(2), 193–225.

    Google Scholar 

  53. Jung, H. Y., Kang, S. M., Kang, Y. M., Kang, M. J., Yun, D. J., Bahk, J. D., Yang, J. K., & Choi, M. S. (2003). Enzyme and Microbial Technology, 33(7), 987–990.

    Article  CAS  Google Scholar 

  54. Liu, Y. H., Liang, Z. S., Chen, B., Yang, D. F., & Liu, J. L. (2009). Enzyme and Microbial Technology, 46, 28–31.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The financial assistance provided by the All India Council for Technical Education (AICTE), Government of India; is duly acknowledged.

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Authors and Affiliations

  1. Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University Hyderabad, Kukatpally, Hyderabad, 500 085, India

    Suryakala Gandi, Kiranmayee Rao, Bhuvaneswari Chodisetti & Archana Giri

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  1. Suryakala Gandi
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  2. Kiranmayee Rao
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Correspondence to Archana Giri.

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Gandi, S., Rao, K., Chodisetti, B. et al. Elicitation of Andrographolide in the Suspension Cultures of Andrographis paniculata . Appl Biochem Biotechnol 168, 1729–1738 (2012). https://doi.org/10.1007/s12010-012-9892-4

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