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Polyamines Induced by Osmotic Stress Protect Synechocystis sp. PCC 6803 Cells and Arginine Decarboxylase Transcripts Against UV-B Radiation

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Abstract

The effect of UV-B radiation on growth and polyamines content of Synechocystis sp. PCC 6803 subjected to either NaCl or sorbitol stress was investigated. Cells could not grow in the presence of 350 mM NaCl or 500 mM sorbitol under normal white light. However, cells grown in BG11 under osmotic stress imposed by NaCl or sorbitol followed by ultraviolet-B (UV-B) irradiation for 2 h showed higher cell density than those under the same condition but no osmotic stress. The chlorophyll fluorescence parameter (F v/F m) also showed an apparent decrease upon UV-B irradiation. Intracellular polyamines increased by about 2- and 4-fold in NaCl- and sorbitol-stressed cells, respectively. When these cells were irradiated with UV-B for 1 h, a further 3-fold increase in polyamines content was detected in NaCl-stressed but not sorbitol-stressed cells. Synechocystis cells contained adc1 and adc2 genes encoding arginine decarboxylase (ADC) with only adc1 showing upregulation by NaCl or sorbitol stress. NaCl- or sorbitol-stressed cells contained about 5-fold higher level of adc1 transcript than did the unstressed cells after 1-h irradiation with UV-B, suggesting the protection of adc1 transcript by accumulated polyamines, due to NaCl or sorbitol stress, against UV-B radiation damage. ADC levels as analyzed by Western blot showed upregulation by UV-B in NaCl-stressed but not sorbitol-stressed cells.

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Acknowledgments

The authors would like to thank Dr. Sorawit Powtongsook (Center of Excellence for Marine Biotechnology, NASTDA, Thailand) for providing the modified Qubit fluorometer. This work was supported by Chulalongkorn University (CU) Graduate School Thesis Grant to A.P. and the TRF-CHE Research Grant for New Scholar, the Thailand Research Fund (TRF) in conjunction with the Commission on Higher Education (CHE), Ministry of Education, Thailand, and Research Funds from the Faculty of Science, CU (A1B1-NS), Chulalongkorn University to S.J. The support from CHE (university staff development consortium) and National Research University Project (FW0659A) to A.I. are also acknowledged.

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Authors and Affiliations

  1. Laboratory of Cyanobacterial Biotechnology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Apiradee Pothipongsa, Saowarath Jantaro & Aran Incharoensakdi

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  1. Apiradee Pothipongsa
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  2. Saowarath Jantaro
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  3. Aran Incharoensakdi
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Correspondence to Saowarath Jantaro.

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Pothipongsa, A., Jantaro, S. & Incharoensakdi, A. Polyamines Induced by Osmotic Stress Protect Synechocystis sp. PCC 6803 Cells and Arginine Decarboxylase Transcripts Against UV-B Radiation. Appl Biochem Biotechnol 168, 1476–1488 (2012). https://doi.org/10.1007/s12010-012-9871-9

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