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Evaluation of Metal Ions and Surfactants Effect on Cell Growth and Exopolysaccharide Production in Two-Stage Submerged Culture of Cordyceps militaris

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Abstract

During the two-stage submerged fermentation of medicinal mushroom Cordyceps militaris, it was found that K+, Ca2+, Mg2+, and Mn2+ were favorable to the mycelial growth. The EPS production reached the highest levels in the media containing Mg2+ and Mn2+. However, Ca2+ and K+ almost failed to increase significantly exopolysaccharides (EPS) production. Sodium dodecyl sulfate (SDS) significantly enhanced EPS production compared with that of without adding SDS when SDS was added on static culture stage of two-stage cultivation process. The presence of Tween 80 in the medium not only simulated mycelial growth but also increased EPS production. By response surface methods (RSM), EPS production reached its peak value of 3.28 g/L under optimal combination of 27.6 mM Mg2+, 11.1 mM Mn2+, and 0.05 mM SDS, which was 3.76-fold compared with that of without metal ion and surfactant. The results obtained were useful in better understanding the regulation for efficient production of EPS of C. militaris in the two-stage submerged culture.

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Acknowledgments

The project was partially supported by the National Natural Science Foundation of China (NSFC, project no. 21072041), open funding project of the National Key Laboratory of Biochemical Engineering (no. KF2010-12) and the Foundation (no. 2012IM004) of Ministry of Education Key Laboratory of Industrial Fermentation Microbiology (Tianjin University of Science and Technology), P. R. China, and Hebei Science Research Projects of Hebei Education Department (no. 2010128).

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Authors and Affiliations

  1. Hebei Key Laboratory of Pharmaceutic Molecular Chemistry, College of Bioscience and Bioengineering, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhang, 050018, People’s Republic of China

    Jian-Dong Cui

  2. Library, Hebei University of Science and Technology, 70 Yuhua East Road, Shijiazhang, 050018, People’s Republic of China

    Ya-Nan Zhang

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  1. Jian-Dong Cui
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  2. Ya-Nan Zhang
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Correspondence to Jian-Dong Cui.

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Cui, JD., Zhang, YN. Evaluation of Metal Ions and Surfactants Effect on Cell Growth and Exopolysaccharide Production in Two-Stage Submerged Culture of Cordyceps militaris . Appl Biochem Biotechnol 168, 1394–1404 (2012). https://doi.org/10.1007/s12010-012-9865-7

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  • DOI: https://doi.org/10.1007/s12010-012-9865-7

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