Skip to main content
SpringerLink
Log in

Solid-Phase Colorimetric Method for the Quantification of Fucoidan

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

We described the simple, selective, and rapid method for determination of fucoidans using methylene blue staining of sulfated polysaccharides, immobilized into filter paper and consequent optic density (at A 663 nm) measurement of the eluted dye from filter paper. This solid-phase method allows selective determination of 1–20 μg fucoidan in presence of potentially interfering compounds (alginic acid, DNA, salts, proteins, and detergents). Further, we demonstrated the alternative way of using image processing software for fucoidan quantification without extraction of methylene blue dye from stained spots of fucoidan–dye complex.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Kylin, H. (1913). Zur Biochemie der meeresalgen. Hoppe-Seyler’s Z Physiol Chem, 83, 171–197.

    Article  CAS  Google Scholar 

  2. Usov, A. I., & Bilan, M. I. (2009). Fucoidans—sulfated polysaccharides of brown algae. Russ Chem Rev, 78, 785–799.

    Article  CAS  Google Scholar 

  3. Farndale, R. W., Buttle, D. J., & Barrett, A. J. (1986). Improved quantification and discrimination of sulfated glycosaminoglycans by use of dimethylmethylene blue. Biochim Biophys Acta, 883, 173–177.

    Article  CAS  Google Scholar 

  4. Soedjak, H. S. (1994). Colorimetric determination of carrageenans and other anionic hydrocolloids with methylene blue. Anal Chem, 66, 4514–4518.

    Article  CAS  Google Scholar 

  5. Liu, Q., & Jiao, Q. (1998). Mechanism of methylene blue action and interference in the heparin assay. Spectros Lett, 31, 913–924.

    Article  CAS  Google Scholar 

  6. Beutlev, J. A., McKee, T. C., Fullew, R. W., Tischler, M., Cardelina, J. H., Snader, K. M., et al. (1993). Frequent occurrence of HIV inhibitory sulfated polysaccharides in marine vertebrates. Antivir Chem Chemother, 4, 167–172.

    Google Scholar 

  7. Albano, R. M., & Mourao, P. A. S. (1986). Isolation, fractionation and preliminary characterization of a novel class of a sulfated glycans from the tunic of Styela plycata (Chondrata Tunicata). J Biol Chem, 261, 758–765.

    CAS  Google Scholar 

  8. Said-Fernandez, S., Gonzalez-Garsa, M. T., Mata-Cardenas, B. D., & Navaro-Marmolejo, L. (1990). A multipurpose solid-phase method for protein determination with Coomassie brilliant blue G-250. Anal Biochem, 191, 119–126.

    Article  CAS  Google Scholar 

  9. Shiroma, R., Konishi, T., Uechi, S., & Tako, M. (2008). Structural study of fucoidan from brown seaweed Hizikia fusiformis. Food Sci Technol Res, 14, 176–182.

    Article  CAS  Google Scholar 

  10. Quatrano, R. S., & Crayton, M. A. (1973). Sulfation of fucoidan in fucus embryos. I. Possible role in localization. Develop Biol, 30, 29–41.

    Article  CAS  Google Scholar 

  11. Irhimeh, M. R., Fitton, J. H., Lowenthal, R. M., & Kongtawelert, P. (2005). A quantitative method to detect fucoidan in human plasma using a novel antibody. Method Find Exp Clin Pharmacol, 27, 705–710.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This research was supported by Fishery Commercialization Support Program Ministry for Food, Agriculture, Forestry and Fisheries, Republic of Korea, grant no 109203-03-3-CG000.

Author information

Authors and Affiliations

  1. Graduate School of Biotechnology, Kyung Hee University, Yongin, Republic of Korea, 446-701

    Jung Min Lee, Z.-U. Shin, Gafurjon T. Mavlonov & Tae-Hoo Yi

  2. Center of Genomic Technologies, Institute of Genetics and Plant Experimental Biology, Academy Sciences of Uzbekistan, Tashkent, Uzbekistan, 111226

    Gafurjon T. Mavlonov & Ibrokhim Y. Abdurakhmonov

Authors
  1. Jung Min Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z.-U. Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gafurjon T. Mavlonov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ibrokhim Y. Abdurakhmonov
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Tae-Hoo Yi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Tae-Hoo Yi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, J.M., Shin, ZU., Mavlonov, G.T. et al. Solid-Phase Colorimetric Method for the Quantification of Fucoidan. Appl Biochem Biotechnol 168, 1019–1024 (2012). https://doi.org/10.1007/s12010-012-9837-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-012-9837-y

Keywords

Search

Navigation