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Extraction and Purification of Purple Membrane for Photochromic Thin Film Development: Application in Photoelectrochemical Investigation

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Abstract

Purple membrane (PM) has been extracted and purified from archaebacteria for thin film development. The purified purple membrane is isolated in 1 % polyvinyl alcohol solution for making thin film within gelatin and organically modified silicate matrices. For thin film within gelatin matrix, homogenized purple membrane suspension is mixed with 8 % gelatin and poured into a specially designed block with desired thickness of spacer having hydrophobicity followed by gelatinization of the same over home-made thermostatic control unit at 38 °C. The gelatinized matrix is then allowed to dry under controlled conditions of humidity and temperature. The films of varying thicknesses ranging between 40, 50, and 60 μ are used for photo-electrochemical measurements. The results on photo-electrochemistry of non-oriented purple membrane film provides valuable information on the generation of forward (light on) and backward (light off) photocurrent as a function of: (a) applied potential and (b) film thickness. An increase in applied negative potential increases the amplitude of photocurrent whereas decrease in film thickness facilitates the reversibility of photocurrent response.

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Acknowledgments

The author is thankful to DST, New Delhi for financial support.

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Authors and Affiliations

  1. Department of Applied Chemistry, Indian Institute of Technology, Banaras Hindu University, Varanasi, 221005, India

    P. C. Pandey, Digvijay Pandey & Richa Singh

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  1. P. C. Pandey
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  2. Digvijay Pandey
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  3. Richa Singh
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Correspondence to P. C. Pandey.

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Pandey, P.C., Pandey, D. & Singh, R. Extraction and Purification of Purple Membrane for Photochromic Thin Film Development: Application in Photoelectrochemical Investigation. Appl Biochem Biotechnol 168, 936–946 (2012). https://doi.org/10.1007/s12010-012-9832-3

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  • DOI: https://doi.org/10.1007/s12010-012-9832-3

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