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Effects of Holdfast of Laminaria japonica on Listeria Invasion on Enterocyte-Like Caco-2 Cells and NO Production of Macrophage RAW 264.7 Cells

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Abstract

Listeria monocytogenes (Lm) causes food poisoning in humans mainly through consumption of ready-to-eat foods. Immunocompromised persons are at the highest risk for infection. We investigated effects of crude soluble polysaccharides (SPS) and ethanolic extract (EE) fractions of frond (kombu) and holdfast (ganiashi) parts of Laminaria japonica on Lm invasion into human enterocyte-like Caco-2 cells and immune and/or inflammatory reactions of murine macrophage RAW 264.7 cells. Recovery and viscosity were high in kombu SPS. Total phenolic content and antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging capacity and Fe-reducing power) were higher in ganiashi EE. EE of ganiashi, rather than kombu, suppressed the Lm invasion into the differentiated Caco-2 cells, though the inhibitory effect of SPS was not significant. Ganiashi SPS increased the nitric oxide (NO) production of intact RAW 264.7 cells. On the other hand, the NO production from Escherichia coli O111 lipopolysaccharide-activated cells was suppressed by kombu SPS and ganiashi EE. These results suggest that L. japonica, particularly ganiashi, might suppress the invasion and infection of Lm and also the inflammation.

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Acknowledgments

This research was supported by the Ministry of Education, Culture, Sports, Science and Technology in Japan, Grant-in-Aid for Scientific Research (C), 22580221, 2010.

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Authors and Affiliations

  1. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Konan, Minato-ku, Tokyo, 108-8477, Japan

    Takashi Kuda, Shinsuke Nakamura, Choa An, Hajime Takahashi & Bon Kimura

  2. Department of Food and Cosmetic Science, Tokyo University of Agriculture, Yasaka, Abashiri, Hokkaido, 099-2493, Japan

    Makoto Nishizawa

Authors
  1. Takashi Kuda
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  2. Shinsuke Nakamura
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  3. Choa An
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  4. Hajime Takahashi
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  5. Bon Kimura
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  6. Makoto Nishizawa
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Correspondence to Takashi Kuda.

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Kuda, T., Nakamura, S., An, C. et al. Effects of Holdfast of Laminaria japonica on Listeria Invasion on Enterocyte-Like Caco-2 Cells and NO Production of Macrophage RAW 264.7 Cells. Appl Biochem Biotechnol 168, 928–935 (2012). https://doi.org/10.1007/s12010-012-9831-4

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  • DOI: https://doi.org/10.1007/s12010-012-9831-4

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