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Immobilization of Pseudomonas fluorescens Lipase onto Magnetic Nanoparticles for Resolution of 2-Octanol

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Abstract

The lipase from Pseudomonas fluorescens (Lipase AK, AKL) was immobilized onto the magnetic Fe3O4 nanoparticles via hydrophobic interaction. Enzyme loading and immobilization yield were determined as 21.4 ± 0.5 mg/g and 49.2 ± 1.8 %, respectively. The immobilized AKL was successfully used for resolution of 2-octanol with vinyl acetate used as acyl donor. Effects of organic solvent, water activity, substrate ratio, and temperature were investigated. Under the optimum conditions, the preferred isomer for AKL is the (R)-2-octanol and the highest enantioselectivity (E = 71.5 ± 2.2) was obtained with a higher enzyme activity (0.197 ± 0.01 μmol/mg/min). The results also showed that the immobilized lipase could be easily separated from reaction media by the magnetic steel and remained 89 % of its initial activity as well as the nearly unchanged enantioselectivity after five consecutive cycles, indicating a high stability in practical operation.

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Abbreviations

AKL:

Lipase from Pseudomonas fluorescens (Lipase AK AKL) was purchased from Amano Pharmaceutical Co. Ltd. (Japan)

a w :

Water activity

E value:

The enantiomeric ratio

ee:

The enantiomeric excess

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Acknowledgments

The authors are grateful for the financial supports from National Natural Science Foundation of China (nos. 31070708, 20772046, and 21172093) and the Natural Science Foundation of Jilin Province (no. 201115038).

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Authors and Affiliations

  1. Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changchun, 130021, People’s Republic of China

    Er-na Xun, Xiao-li Lv, Wei Kang, Jia-xin Wang, Hong Zhang, Lei Wang & Zhi Wang

  2. College of Stomatology, Jilin University, Changchun, 130041, People’s Republic of China

    Xiao-li Lv

  3. Tianjin Vocational Biological Engineering Technical College, Tianjin developing west district, Tianjin, 300462, People’s Republic of China

    Wei Kang

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  1. Er-na Xun
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  2. Xiao-li Lv
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  5. Hong Zhang
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Correspondence to Lei Wang or Zhi Wang.

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Xun, En., Lv, Xl., Kang, W. et al. Immobilization of Pseudomonas fluorescens Lipase onto Magnetic Nanoparticles for Resolution of 2-Octanol. Appl Biochem Biotechnol 168, 697–707 (2012). https://doi.org/10.1007/s12010-012-9810-9

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  • DOI: https://doi.org/10.1007/s12010-012-9810-9

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