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Hydrogen and Methane Production, Energy Recovery, and Organic Matter Removal from Effluents in a Two-Stage Fermentative Process

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Abstract

This study evaluates the potential for using different effluents for simultaneous H2 and CH4 production in a two-stage batch fermentation process with mixed microflora. An appreciable amount of H2 was produced from parboiled rice wastewater (23.9 mL g−1 chemical oxygen demand [COD]) and vinasse (20.8 mL g−1 COD), while other effluents supported CH4 generation. The amount of CH4 produced was minimum for sewage (46.3 mL g−1 COD), followed by parboiled rice wastewater (115.5 mL g−1 COD) and glycerol (180.1 mL g−1 COD). The maximum amount of CH4 was observed for vinasse (255.4 mL g−1 COD). The total energy recovery from vinasse (10.4 kJ g−1 COD) corresponded to the maximum COD reduction (74.7 %), followed by glycerol (70.38 %, 7.20 kJ g−1 COD), parboiled rice wastewater (63.91 %, 4.92 kJ g−1 COD), and sewage (51.11 %, 1.85 kJ g−1 COD). The relatively high performance of vinasse in such comparisons could be attributed to the elevated concentrations of macronutrients contained in raw vinasse. The observations are based on kinetic parameters of H2 and CH4 production and global energy recovery of the process. These observations collectively suggest that organic-rich effluents can be deployed for energy recovery with sequential generation of H2 and CH4.

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Abbreviations

COD:

chemical oxygen demand, in milligrams per liter

BOD:

biological oxygen demand, in milligrams per liter

HRT:

hydraulic retention time, in hours

C:

carbon

N:

nitrogen

P:

phosphorus

TS:

total solids, in milligrams per liter

VS:

volatile solids, in milligrams per liter

FS:

fixed solids, in milligrams per liter

VSS:

volatile suspended solids, in milligrams per liter

X :

microorganisms concentration, in milligrams per liter VSS

S:

substrate, in milligrams per liter

SMP:

soluble metabolites production, in milligrams per liter

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Acknowledgments

The authors wish to thank Murilo Daniel de Mello Innocentini and Tatiane Lotufo Leite for providing some of the industrial effluents used in this work. This work was funded by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil (CNPq).

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Authors and Affiliations

  1. Laboratory of Biological Processes, Center for Research, Development and Innovation in Environmental Engineering, São Carlos School of Engineering, University of Sao Paulo, João Dagnone, 1100 Santa Angelina, CEP 13563-120, São Carlos, Brazil

    Guilherme Peixoto, Jorge Luis Rodrigues Pantoja-Filho, José Augusto Bolzan Agnelli & Marcelo Zaiat

  2. Department of Chemical Engineering, Federal University of Triângulo Mineiro, Frei Paulino 30, Abadia, CEP 38025-180, Uberaba, Brazil

    Marlei Barboza

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  1. Guilherme Peixoto
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Correspondence to Guilherme Peixoto.

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Peixoto, G., Pantoja-Filho, J.L.R., Agnelli, J.A.B. et al. Hydrogen and Methane Production, Energy Recovery, and Organic Matter Removal from Effluents in a Two-Stage Fermentative Process. Appl Biochem Biotechnol 168, 651–671 (2012). https://doi.org/10.1007/s12010-012-9807-4

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