Abstract
The interaction between papain and two typical ionic liquids (ILs), 1-octyl-3-methylimidazolium chloride ([C8mim]Cl) and 1-butyl-3-methylimidazolium chloride ([C4mim]Cl), was investigated by using fluorescence spectroscopy technique at a pH value of 7.4. The results suggested that ILs could quench the intrinsic fluorescence of papain probably via a static quenching mechanism. The binding constants were determined by employing the fluorescence quenching method. They were very small compared with that of volatile organic solvents, indicating that only very weak interaction between ILs and papain existed. The Gibbs free energy change (∆G), enthalpy change (∆H), and entropy change (∆S) during the interaction of papain and ILs were estimated. Negative values of these parameters indicated that the interaction between ILs and papain was a spontaneous process, also implying that hydrogen bonding and van der Waals forces played important roles in the interaction processes.
Similar content being viewed by others
References
Kotadia, D. A., & Soni, S. S. (2012). Journal of Molecular Catalysis A: Chemical, 353–354, 44–49.
Banerjee, S., Adak, L., & Ranu, B. C. (2012). Tetrahedron Letters, 53, 2149–2152.
Yang, Q., Xing, H., Su, B., Yu, K., Bao, Z., Yang, Y., et al. (2012). Chemical Engineering Journal, 181–182, 334–342.
Tonova, K. (2012). Separation and Purification Technology, 89, 57–65.
Fang, Y., Zhang, J., Zhou, X., Lin, Y., & Fang, S. (2012). Electrochimica Acta, 68, 235–239.
Yoon, J., Kang, D., Won, J., Park, J. Y., & Kang, Y. S. (2012). Journal of Power Sources, 201, 395–401.
Ma, J. M., Cai, L. L., Zhang, B. J., Hu, L. W., Li, X. Y., & Wang, J. J. (2010). Ecotoxicology and Environmental Safety, 73, 1465–1469.
Studzińska, S., & Buszewski, B. (2009). Analytical and Bioanalytical Chemistry, 393, 983–990.
Costello, D. M., Brown, L. M., & Lamberti, G. A. (2009). Green Chemistry, 11, 548–553.
Thomas, M. F., Li, L. L., Handley-Pendleton, J. M., van der Lelie, D., Dunn, J. J., & Wishart, J. F. (2011). Bioresource Technology, 102, 11200–11203.
Akdogan, Y., Junk, M. J. N., & Hinderberger, D. (2011). Biomacromolecules, 12, 1072–1079.
Rodrigues, J. V., Prosinecki, V., Marrucho, I., Rebelo, L. P. N., & Gomes, C. M. (2011). Physical Chemistry Chemical Physics, 13, 13614–13616.
Lou, W. Y., Zong, M. H., Smith, T. J., Wu, H., & Wang, J. F. (2006). Green Chemistry, 8, 509–512.
Ou, G., Yang, J., He, B., & Yuan, Y. (2011). Journal of Molecular Catalysis B: Enzymatic, 68, 66–70.
Cardoso, L., & Micaelo, N. M. (2011). ChemPhysChem, 12, 275–277.
Wang, H., Wang, J., & Zhang, S. (2011). Physical Chemistry Chemical Physics, 13, 3906–3910.
Ding, Y., Zhang, L., Xie, J., & Guo, R. (2010). Journal of Physical Chemistry B, 114, 2033–2043.
Szabó, A., Kotormán, M., Laczkó, I., & Simon, L. M. (2006). Journal of Molecular Catalysis B: Enzymatic, 41, 43–48.
Liu, P., Tian, G., Lee, K. S., Wong, M. S., & Ye, Y. (2002). Tetrahedron Letters, 43, 2423–2425.
Fan, Y., Zhang, S., Kong, J., & Miao, J. (2011). Microchemical Journal, 99, 439–442.
Fan, J., Fan, Y., Zhang, S., & Wang, J. (2011). Separation Science and Technology, 46, 1172–1177.
He, L., Wang, X., Liu, B., Wang, J., Sun, Y., Gao, E., et al. (2011). Journal of Luminescence, 131, 285–290.
Hossain, M., Khan, A. Y., & Kumar, G. S. (2012). The Journal of Chemical Thermodynamics, 47, 90–99.
Cui, F., Zhang, Q., Yao, X., Luo, H., Yang, Y., Qin, L., et al. (2008). Pesticide Biochemistry and Physiology, 90, 126–134.
Wang, J. T., Wu, Q. H., Wang, C., Ma, J. J., Zang, X. H., Yang, X. M., et al. (2008). Spectroscopy Letters, 41, 128–135.
Yuan, H., Ranatunga, R., Carr, P. W., & Pawliszyn, J. (1999). The Analyst, 124, 1443–1448.
Pham, T. P. T., Cho, C. W., & Yun, Y. S. (2010). Water Research, 44, 352–372.
Bi, S., Yan, L., Pang, B., & Wang, Y. (2012). Journal of Luminescence, 132, 132–140.
Xiao, Q., Huang, S., Liu, Y., Tian, F. F., & Zhu, J. C. (2009). Journal of Fluorescence, 19, 317–326.
Zhang, Y. Z., Zhang, J., Li, F. F., Xiang, X., Ren, A. Q., & Liu, Y. (2011). Molecular Biology Reports, 38, 2445–2453.
Zhang, L. N., Wu, F. Y., & Liu, A. H. (2011). Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 79, 97–103.
Lu, Y., Feng, Q., Cui, F., Xing, W., Zhang, G., & Yao, X. (2010). Bioorganic & Medicinal Chemistry Letters, 20, 6899–6904.
Zhang, Z., & Tang, R. (2012). Journal of Molecular Structure, 1010, 116–122.
Ross, P. D., & Subramanian, S. (1981). Biochemistry, 20, 3096–3102.
Dong, K., Zhang, S., Wang, D., & Yao, X. (2006). The Journal of Physical Chemistry A, 110, 9775–9782.
Fumino, K., Peppel, T., Geppert-Rybczyńska, M., Zaitsau, D. H., Lehmann, J. K., Verevkin, S. P., et al. (2011). Physical Chemistry Chemical Physics, 13, 14064–14075.
Tomé, L. I. N., Jorge, M., Gomes, J. R. B., & Coutinho, J. A. P. (2012). The Journal of Physical Chemistry B, 116, 1831–1842.
Tomé, L. I. N., Domínguez-Pérez, M., Cláudio, A. F. M., Freire, M. G., Marrucho, I. M., Cabeza, O., et al. (2009). The Journal of Physical Chemistry B, 113, 13971–13979.
Pan, X., Qin, P., Liu, R., & Wang, J. (2011). Journal of Agricultural and Food Chemistry, 59, 6650–6656.
Acknowledgments
This work was financially supported by the Natural Science Foundation of the Education Department of Henan Province (Nos. 12B150017 and 12A150010) and the Foundation of Henan Polytechnic University (No. B2010-14).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Fan, Y., Yan, J., Zhang, S. et al. Fluorescence Spectroscopic Analysis of the Interaction of Papain with Ionic Liquids. Appl Biochem Biotechnol 168, 592–603 (2012). https://doi.org/10.1007/s12010-012-9801-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-012-9801-x