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Role of TDZ in the Quick Regeneration of Multiple Shoots from Nodal Explant of Vitex trifolia L.—an Important Medicinal Plant

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Abstract

The effect of thidiazuron (TDZ) has been investigated in shoot multiplication for a simple, efficient, rapid, and commercially applicable regeneration protocol of an important medicinal plant, Vitex trifolia. Multiple shoots were induced in nodal explants obtained from a mature tree on Murashige and Skoog (MS) medium supplemented with TDZ in various concentrations (0.5, 1.0, 2.5, 5.0, 7.5, or 10.0 μM). Prolonged exposure of the culture to TDZ had an adverse affect. To avoid this, the cultures were transferred to TDZ-free MS medium or MS medium fortified with various concentrations of 6-benzyladenine (BA) alone or in combination with α-naphthalene acetic acid (NAA) to enhance multiplication, proliferation, and elongation of induced shoots. Optimum shoot multiplication and elongation was achieved when TDZ-exposed explants were repeatedly subcultured on MS media containing a combination of 1.0 μM BA and 0.5 μM NAA. The highest shoot regeneration frequency (90 %) and maximum number (22.3 ± 0.2) of shoots per explant with shoot length of (5.2 ± 0.2 cm) was recorded on MS medium fortified with 5.0 μM TDZ. In vitro rooting of isolated shoots was achieved best in half-strength MS medium containing 0.5 μM NAA. Properly rooted plantlets were successfully hardened off and acclimatized in thermocol cups containing sterile Soilrite. These plantlets were then transferred to pots containing different potting substrate; percentage survival of the plantlets was highest in vermiculite/garden soil mixture (1:1) and successfully transfer to greenhouse under sunlight.

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Acknowledgments

Md. Rafique Ahmed is thankful to the University Grants Commission (UGC), Govt. of India, New Delhi for its award of Junior Research Fellow (F1-17.1/2011/MANF-MUS-MAN-581) (SA-III/manfugc). Research support provided by the Department of Science and Technology (DST) in the form of DST-FIST (2011-16) and UGC in the form of UGC-SAP; DRS-I Programme is duly acknowledged.

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Authors and Affiliations

  1. Plant Biotechnology Laboratory, Department of Botany, Aligarh Muslim University, Aligarh, 202 002, India

    Md Rafique Ahmed & Mohammad Anis

  2. Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, PO Box 2460, Riyadh, 11451, Saudi Arabia

    Mohammad Anis

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  1. Md Rafique Ahmed
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  2. Mohammad Anis
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Correspondence to Mohammad Anis.

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Ahmed, M.R., Anis, M. Role of TDZ in the Quick Regeneration of Multiple Shoots from Nodal Explant of Vitex trifolia L.—an Important Medicinal Plant. Appl Biochem Biotechnol 168, 957–966 (2012). https://doi.org/10.1007/s12010-012-9799-0

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  • DOI: https://doi.org/10.1007/s12010-012-9799-0

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