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Effects of Inoculum Physiological Stage on the Growth Characteristics of Chlorella sorokiniana Cultivated Under Different CO2 Concentrations

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Abstract

In order to maximize microalgae biomass production and reduce its overall costs, it is important to optimize inoculum conditions based on its physical and physiological characteristics. Chlorella sorokiniana cultures inoculated with inoculum at three different physiological stages (lag, exponential, and stationary) diluted to the same optical density were cultivated for 12 days under three different CO2 concentrations (0.038, 5, or 10 % CO2 v/v) and growth pattern and biomass production was observed. Samples inoculated with lag phase inoculum supplied with 5 % CO2 achieved the maximum biomass production, whereas samples supplied with 0.038 % CO2 never reached exponential growth. The better growth of samples inoculated with lag phase inoculum was attributed to its increased number of cells compared to the other two inocula.

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Acknowledgments

This work was partially funded by grants and subcontracts from the US DOE and the State of Georgia. The authors acknowledge the assistance of other members of the Biorefining and Carbon Cycling Program's Algae-Biofuels Lab in analytical analysis.

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Authors and Affiliations

  1. College of Engineering, The University of Georgia, Athens, GA, 30602, USA

    Erico R. Mattos, Manjinder Singh & Keshav C. Das

  2. Department of Crop and Soil Sciences, The University of Georgia, Athens, GA, 30602, USA

    Miguel L. Cabrera

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  1. Erico R. Mattos
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  2. Manjinder Singh
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  3. Miguel L. Cabrera
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  4. Keshav C. Das
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Biorefining and Carbon Cycling Program

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Correspondence to Keshav C. Das.

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Mattos, E.R., Singh, M., Cabrera, M.L. et al. Effects of Inoculum Physiological Stage on the Growth Characteristics of Chlorella sorokiniana Cultivated Under Different CO2 Concentrations. Appl Biochem Biotechnol 168, 519–530 (2012). https://doi.org/10.1007/s12010-012-9793-6

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  • DOI: https://doi.org/10.1007/s12010-012-9793-6

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