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Adaptation of Glycolysis and Growth to Acetate in Sporolactobacillus sp. Y2-8

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Abstract

Exogenous addition of a low concentration of acetate (2 g/L sodium acetate) effectively decreased acetic acid excretion and lowered the ATP content in Sporolactobacillus sp. Y2-8 without any growth defect although the acetate could not be utilized at an initial glucose concentration of 150 g/L. This induced an enhanced glycolytic flux with increased specific activities of hexokinase and phosphofructokinase, probably to compensate for the lowered efficiency of ATP production. However, with increasing concentrations (5 g/L sodium acetate), acetate was utilized first before being produced again, causing a growth lag at the transition. Glucose consumption was also reduced at high acetate concentrations, resulting in decreased d-lactic acid production. These results demonstrate that acetate plays a significant role in regulating glycolysis and growth of Sporolactobacillus.

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Acknowledgments

This work was supported by a grant from the National Outstanding Youth Foundation of China (Grant No.: 21025625); the National High-Tech Research and Development Program of China (863) (Grant No.: 2012AA021203); the National Basic Research Program of China (973) (Grant No.: 2011CBA00806); the National Natural Science Foundation of China, Youth Program (Grant No.:21106070); the Changjiang Scholars and Innovative in University (PCSIRT), and Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Life Science and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, 210009, Peoples’ Republic of China

    Dong Liu, Yong Chen, An Li, Ting Zhao, Tao Zhou, Bingbing Li, Jingjing Xie, Xiaochun Chen, Jianxin Bai, Yanan Hu, Bingfang He & Hanjie Ying

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  1. Dong Liu
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  2. Yong Chen
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  3. An Li
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  5. Tao Zhou
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  12. Hanjie Ying
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Correspondence to Hanjie Ying.

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Dong Liu and Yong Chen equally contributed to this study.

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Liu, D., Chen, Y., Li, A. et al. Adaptation of Glycolysis and Growth to Acetate in Sporolactobacillus sp. Y2-8. Appl Biochem Biotechnol 168, 455–463 (2012). https://doi.org/10.1007/s12010-012-9789-2

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  • DOI: https://doi.org/10.1007/s12010-012-9789-2

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