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An Improved Process of Ethanol Production from Hemicellulose: Bioconversion of Undetoxified Hemicellulosic Hydrolyzate from Steam-Exploded Corn Stover with a Domesticated Pichia stipitis

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Abstract

Bioconversion of undetoxified hemicellulosic hydrolyzate from steam-exploded corn stover was investigated with a domesticated Pichia stipitis CBS 5776. The countercurrent washing was applied to recover sugars from the steam-exploded corn stover, which could enrich sugars in washing liquor and give an efficient saving of water. Acid concentration, reaction temperature, and time were optimized for the acid post-hydrolysis of oligosaccharides in steam-exploded prehydrolyzate by a central composite design and response surface methodology. The domestication of P. stipitis to the hydrolyzate resulted in improving sugar consumption and ethanol yield by gradually increasing the ratio of hydrolyzate in the medium. Recycling utilization of the domesticated yeast demonstrated that the yeast kept a stable ability of fermenting both hexose and pentose in the undetoxified hydrolyzate. The sugar consumption and ethanol yield were over 90 and 80 %, respectively.

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Acknowledgments

The authors acknowledge the financial support from the National Natural Science Foundation of China (grant nos. 31000278 and 31070523), Research Fund for the Doctoral Program of Higher Education of China (grant no. 20103204120009), Governmental Public Industry Research Special Funds for Projects (grant no. 201004001), and The Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Authors and Affiliations

  1. College of Chemical Engineering, Nanjing Forestry University, No. 159 Long Pan Road, Nanjing, 210037, Jiangsu Province, China

    Qiang Yong, Xin Li, Yun Yuan, Chenhuan Lai, Nannan Zhang, Qiulu Chu, Yong Xu & Shiyuan Yu

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  1. Qiang Yong
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  2. Xin Li
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  3. Yun Yuan
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  4. Chenhuan Lai
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  5. Nannan Zhang
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  6. Qiulu Chu
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  7. Yong Xu
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  8. Shiyuan Yu
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Correspondence to Qiang Yong.

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Yong, Q., Li, X., Yuan, Y. et al. An Improved Process of Ethanol Production from Hemicellulose: Bioconversion of Undetoxified Hemicellulosic Hydrolyzate from Steam-Exploded Corn Stover with a Domesticated Pichia stipitis . Appl Biochem Biotechnol 167, 2330–2340 (2012). https://doi.org/10.1007/s12010-012-9778-5

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  • DOI: https://doi.org/10.1007/s12010-012-9778-5

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