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Characterization of Bioimprinted Tannase and Its Kinetic and Thermodynamics Properties in Synthesis of Propyl Gallate by Transesterification in Anhydrous Medium

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Abstract

Tannase has been extensively applied to synthesize gallic acid esters. Bioimprinting technique can evidently enhance transesterification-catalyzing performance of tannase. In order to promote the practical utilization of the modified tannase, a few enzymatic characteristics of the enzyme and its kinetic and thermodynamics properties in synthesis of propyl gallate by transesterification in anhydrous medium have been studied. The investigations of pH and temperature found that the imprinted tannase holds an optimum activity at pH 5.0 and 40 °C. On the other hand, the bioimprinting technique has a profound enhancing effect on the adapted tannase in substrate affinity and thermostability. The kinetic and thermodynamic analyses showed that the modified tannase has a longer half-time of 1,710 h at 40 °C; the kinetic constants, the activation energy of reversible thermal inactivation, and the activation energy of irreversible thermal inactivation, respectively, are 0.054 mM, 17.35 kJ mol−1, and 85.54 kJ mol−1 with tannic acid as a substrate at 40 °C; the free energy of Gibbs (ΔG) and enthalpy (ΔH) were found to be 97.1 and 82.9 kJ mol-1 separately under the same conditions.

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Acknowledgments

This work has been supported by grants Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-YW-G-050) and the National High Technology Research and Development Program of China (SQ2008AA02Z4477854).

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Authors and Affiliations

  1. Key Lab of Ion Beam Bioengineering, Chinese Academy of Science, 230031, Hefei, China

    Guangjun Nie, Zhiming Zheng, Guohong Gong, Genhai Zhao, Yan Liu, Junying Song & Jun Dai

  2. College of Biochemical Engineering, Anhui Polytechnic University, 241000, Wuhu, China

    Guangjun Nie & Yan Liu

  3. School of Life Science, University of Science and Technology of China, 230022, Hefei, China

    Guangjun Nie

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  1. Guangjun Nie
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Correspondence to Zhiming Zheng.

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Nie, G., Zheng, Z., Gong, G. et al. Characterization of Bioimprinted Tannase and Its Kinetic and Thermodynamics Properties in Synthesis of Propyl Gallate by Transesterification in Anhydrous Medium. Appl Biochem Biotechnol 167, 2305–2317 (2012). https://doi.org/10.1007/s12010-012-9775-8

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