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Heat Shock Treatment Improves Trametes versicolor Laccase Production

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Abstract

An efficient heat shock strategy has been developed to improve laccase production in submerged Trametes versicolor cultures. The optimized heat shock strategy consists of subjecting T. versicolor mycelial pellets to three heat shock treatments at 45 °C for 45 min, starting at culture day 0, with a 24-h interval between treatments. Laccase production increased by more than 1.6-fold relative to the control in both flasks and a 5-L bioreactor because the expression of the laccase gene was enhanced by heat shock induction. The present work demonstrates that heat shock induction is a promising method because it both improves fungal laccase production and has a good potential in industrial application.

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Acknowledgment

This work was financially supported by the National Natural Science Foundation of China (no. 21176241), the Beijing Natural Science Foundation (no. 2102041), the National Key Technology Research and Development Program of China (no. 2012BAK25B01), and the Knowledge Innovation Program of the Chinese Academy of Sciences (nos. KSCX1-YW-11-D1 and KGCX2-YW-337). The authors thank Dr. Amanda R. Stiles (the Chinese Academy of Sciences Fellowships for Young International Scientists, no. 2011Y1GA01) for her assistance in editing the manuscript.

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Authors and Affiliations

  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, People’s Republic of China

    Feng Wang, Chen Guo & Chun-Zhao Liu

  2. College of Arts & Science, Beijing Union University, Beijing, 100191, People’s Republic of China

    Tao Wei & Tian Zhang

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  1. Feng Wang
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  2. Chen Guo
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  5. Chun-Zhao Liu
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Correspondence to Chun-Zhao Liu.

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Wang, F., Guo, C., Wei, T. et al. Heat Shock Treatment Improves Trametes versicolor Laccase Production. Appl Biochem Biotechnol 168, 256–265 (2012). https://doi.org/10.1007/s12010-012-9769-6

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  • DOI: https://doi.org/10.1007/s12010-012-9769-6

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