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Simple Strategy of Repeated Batch Cultivation for Enhanced Production of 1,3-Propanediol Using Clostridium diolis

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Abstract

Repeated batch cultivation (empty-and-fill protocol) using obligate anaerobe Clostridium diolis was attempted in the present study to improve the production of 1,3-propanediol (1,3-PD). In repeated batch operation, 20 % (v/v) culture broth was removed from the bioreactor and supplemented with an equal volume of fresh nutrient medium when the residual glycerol concentration in the bioreactor decreased below 15 g/L. Four cycles of culture broth withdrawal and subsequent replacement resulted in achieving a 1,3-PD concentration of 67.8 g/L with a productivity of 1.04 g/L/h at the end of 65 h. This represented a 1,3-PD concentration and productivity enhancement by 2.6-fold and 1.5-fold, respectively, as compared to batch 1,3-PD fermentation. This is the first report on the use of repeated batch mode of bioreactor operation for enhanced 1,3-PD production.

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Acknowledgments

The Senior Research Fellowship (SRF) award by Indian Council of Medical Research (ICMR), Govt. of India, New Delhi for the execution of the project is gratefully acknowledged by one of the authors (Ms. Guneet Kaur).

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Authors and Affiliations

  1. Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India

    Guneet Kaur, Ashok K. Srivastava & Subhash Chand

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  1. Guneet Kaur
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  2. Ashok K. Srivastava
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  3. Subhash Chand
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Correspondence to Ashok K. Srivastava.

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Kaur, G., Srivastava, A.K. & Chand, S. Simple Strategy of Repeated Batch Cultivation for Enhanced Production of 1,3-Propanediol Using Clostridium diolis . Appl Biochem Biotechnol 167, 1061–1068 (2012). https://doi.org/10.1007/s12010-012-9715-7

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