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Amperometric Vitamin C Biosensor Based on the Immobilization of Ascorbate Oxidase into the Biocompatible Sandwich-Type Composite Film

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Abstract

Ascorbate oxidase (AO), a biologically active macromolecule, was successfully immobilized into a biocompatible sandwich-type composite film for developing the vitamin C (VC) biosensor, and the content of VC in commercial juices was amperometrically determined. The biocompatible and conducting poly(3,4-ethylenedioxythiophene) composite film and highly stable and selective multiwalled carbon nanotubes –Nafion composite film were prepared as inner and outer films of biosensor. AO molecules were immobilized between these two composite films. The as-fabricated biosensor displayed an excellent bioelectrocatalytic performance towards the oxidation of VC, a fast current response, a low working potential, a high sensitivity, a wide linear range, and a low detection limit. Moreover, the working mechanism of the biosensor was proposed, and its kinetics was also discussed. In addition, the specificity, reproducibility, and feasibility of the as-fabricated biosensor were also evaluated. Good results of the VC determination in commercial juices indicated that the as-fabricated biosensor was a potential candidate for the electrochemical determination of VC in agricultural crops. Inner and outer films provided a promising platform for the immobilization of biologically active species.

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Acknowledgments

NSFC (50963002 and 51073074), Key Projects in the National Science & Technology Pillar Program in the Eleventh Five-Year Plan Period (2006BAD02A04, 2006BAD01A01), Jiangxi Provincial Department of Science and Technology (2006BAD01A01-2-5), Jiangxi Provincial Department of Education (GJJ11590 and GJJ10678), Natural Science Foundation of Jiangxi Province (2010GZH0041), and Key Laboratory of Photochemical Conversion and Optoelectronic Materials, TIPC, are acknowledged for their financial support.

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Authors and Affiliations

  1. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, 330013, People’s Republic of China

    Yangping Wen, Jingkun Xu, Ming Liu & Dong Li

  2. Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, and Key Laboratory of Physiology, Ecology and Cultivation of Double Cropping Rice, Ministry of Agriculture, College of Agronomy, Jiangxi Agricultural University, Nanchang, 330045, People’s Republic of China

    Yangping Wen, Ming Liu, Dong Li & Haohua He

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  1. Yangping Wen
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Correspondence to Jingkun Xu or Haohua He.

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Wen, Y., Xu, J., Liu, M. et al. Amperometric Vitamin C Biosensor Based on the Immobilization of Ascorbate Oxidase into the Biocompatible Sandwich-Type Composite Film. Appl Biochem Biotechnol 167, 2023–2038 (2012). https://doi.org/10.1007/s12010-012-9711-y

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  • DOI: https://doi.org/10.1007/s12010-012-9711-y

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