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Applied Biochemistry and Biotechnology

, Volume 167, Issue 2, pp 270–284 | Cite as

Isolation, Screening, and Optimization of the Fermentation Conditions of Highly Cellulolytic Bacteria from the Hindgut of Holotrichia parallela Larvae (Coleoptera: Scarabaeidae)

  • Ping Sheng
  • Shengwei Huang
  • Qi Wang
  • Ailing Wang
  • Hongyu ZhangEmail author
Article

Abstract

From the hindgut contents of Holotrichia parallela, 93 cellulolytic bacterial isolates were isolated after enrichment in carboxymethyl cellulose medium. Among these isolates, a novel bacterium, designated HP207, with the highest endoglucanase productivity was selected for further study. This bacterium was identified as Pseudomonas sp. based on the results of the 16S ribosomal DNA analysis, morphological characteristics, and biochemical properties. The production of the endoglucanase was optimized by varying various physical culture conditions using a submerged fermentation method. Under the optimized fermentation conditions, the maximum endoglucanase activity of 1.432 U mL−1 in bacterial cultures was obtained, higher than those of the most widely studied bacteria and fungi, which are the attractive candidates for the commercial producer of cellulase. And the crude endoglucanase enzyme was also highly thermostable; approximately 55 % of the original activity was maintained after pretreatment at 70 °C for 1 h. Thus, from the present study, the bacterium can be added up to the database of cellulolytic bacteria.

Keywords

Holotrichia parallela Cellulolytic bacteria Pseudomonas sp. HP207 Endoglucanase 

Notes

Acknowledgments

This research was supported by the National Natural Science Foundation of China (no. 30671404), the Special Fund for Agro-scientific Research in the Public Interest (no. 201003025), the earmarked fund for Modern Agro-industry Technology Research System of China (no. CARS-27), and the Specialized Research Fund for the Doctoral Program of Higher Education of China (no. 200805040010). The authors thank Xiaoxue Li and Cong Li for comments on the manuscript.

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Ping Sheng
    • 1
  • Shengwei Huang
    • 1
  • Qi Wang
    • 1
  • Ailing Wang
    • 1
  • Hongyu Zhang
    • 1
    Email author
  1. 1.State Key Laboratory of Agricultural Microbiology, Institute of Urban and Horticultural Pests, and Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

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