Advertisement

Applied Biochemistry and Biotechnology

, Volume 167, Issue 2, pp 259–269 | Cite as

Immunogenicity of Japanese Encephalitis Virus Envelope Protein by Hyphantria cunea Nuclear Polyhedrosis Virus Vector in Guinea Pig

  • Hyung-Hoan Lee
  • Seung-Kuk Hong
  • Sang-Ho Yoon
  • Sung-Jae Jang
  • Young-Yil Bahk
  • Min-Dong Song
  • Pyo-Jam Park
  • Kwang-Ho Lee
  • Chan-Gil Kim
  • Bokyung Kim
  • Tae-Kyu Park
  • Hyun KangEmail author
Article

Abstract

Japanese encephalitis virus (JEV) is an important pathogen causing febrile syndrome, encephalitis, and death. Envelop (E) glycoprotein is the major target of inducing neutralizing antibodies and protective immunity in host. In this study, E glycoprotein of JEV was expressed in Spodoptera frugiperd 9 cells as a fusion protein containing a gX signal sequence of pseudorabies virus. This purified HcE recombinant protein was evaluated for their immunogenicity and protective efficacy in guinea pig. The survival rates of guinea pig immunized with HcE protein was significantly increased over that of JE vaccine. This result indicates helpful information for developing a subunit vaccine against JEV.

Keywords

Hyphantria cunea envelope (HcE) Japanese encephalitis virus (JEV) Envelop (E) protein Protective immunity 

Notes

Acknowledgment

This work was supported by Konkuk University in 2012.

References

  1. 1.
    Burke, D. S. & Monath, T. P. (2001) Flaviviridae, Fields Virology, 5th Edition, Lippincott Williams & Wilkins, Philadelphia, 1:1043–1125Google Scholar
  2. 2.
    Chen, H. W., Pan, C. H., et al. (1999). Screening of protective antigens of Japanese encephalitis virus by DNA immunization: a comparative study with conventional viral vaccines. Journal of Virology, 73(12), 10137–10145.Google Scholar
  3. 3.
    Kaur, R., Sachdeva, G., et al. (2002). Plasmid DNA immunization against Japanese encephalitis virus: immunogenicity of membrane-anchored and secretory envelope protein. Journal of Infectious Diseases, 185(1), 1–12.CrossRefGoogle Scholar
  4. 4.
    Konishi, E., Mason, P. W., et al. (1996). Enzyme-linked immunosorbent assay using recombinant antigens for serodiagnosis of Japanese encephalitis. Journal of Medical Virology, 48(1), 76–79.CrossRefGoogle Scholar
  5. 5.
    Kwon, H. J., Kang, B. J., et al. (1976). Studies of Japanese encephalitis live virus vaccine.m. Pathogenicity of tissue culture attenuated strain of virus (Anyang strain). Research Report Office Rural Development, 18, 21–28.Google Scholar
  6. 6.
    Lee, H. H., Kang, H., et al. (1999). Construction of a transfer vector contaning gX signal sequence of Pseudorabies virus and a recombinant Baculovirus. Journal of Microbiology and Biotechnology, 9, 541–547.Google Scholar
  7. 7.
    Matsuura, Y., Miyamoto, M., et al. (1989). Characterization of Japanese encephalitis virus envelope protein expressed by recombinant baculoviruses. Virology, 173(2), 674–682.CrossRefGoogle Scholar
  8. 8.
    McAda, P. C., Mason, P. W., et al. (1987). Partial nucleotide sequence of the Japanese encephalitis virus genome. Virology, 158(2), 348–360.CrossRefGoogle Scholar
  9. 9.
    Park, K. J., Kang, B. J.,et. al. (1993). Sequence analysis of polyhedrin gene promoter and construction of an expression vector of hyphantria cunea n uc1ear polyhedrosis virus. Journal of the Korean Society of Virology, 23(2), 141–151.Google Scholar
  10. 10.
    Russell, P. K., W. E., et. al. (1980) Chemical and antigenic structure of flaviviruses, Academic Press, New York, 1:503–529.Google Scholar
  11. 11.
    Seif, I., Coulon, P., et al. (1985). Rabies virulence: effect on pathogenicity and sequence characterization of rabies virus mutations affecting antigenic site III of the glycoprotein. Journal of Virology, 53(3), 926–934.Google Scholar
  12. 12.
    Sumiyoshi, H., Mori, C., et al. (1987). Complete nucleotide sequence of the Japanese encephalitis virus genome RNA. Virology, 161(2), 497–510.CrossRefGoogle Scholar
  13. 13.
    Tsai, T.F.; Chang G.J.J. et. al., (1999) Japanese encephalitis vaccines, Vaccines, 3rd Edition, W.B. Saunders Company, Pennysylvania, 1:672–710.Google Scholar
  14. 14.
    Wengler, G., Castle, E., et al. (1985). Sequence analysis of the membrane protein V3 of the flavivirus West Nile virus and of its gene. Virology, 147(2), 264–274.CrossRefGoogle Scholar
  15. 15.
    Westaway, E. G. (1987). Flavivirus replication strategy. Advances in Virus Research, 33, 45–90.CrossRefGoogle Scholar
  16. 16.
    World Health Organization. (1998). Japanese encephalitis vaccine. Weekly Epidermiological Records, 73, 337–344.Google Scholar
  17. 17.
    Wright, P. J. (1982). Envelope protein of the flavivirus Kunjin is apparently not glycosylated. Journal of General Virology, 59(Pt 1), 29–38.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  • Hyung-Hoan Lee
    • 1
    • 2
  • Seung-Kuk Hong
    • 1
    • 2
  • Sang-Ho Yoon
    • 1
  • Sung-Jae Jang
    • 1
  • Young-Yil Bahk
    • 1
  • Min-Dong Song
    • 1
  • Pyo-Jam Park
    • 1
  • Kwang-Ho Lee
    • 1
  • Chan-Gil Kim
    • 1
  • Bokyung Kim
    • 1
  • Tae-Kyu Park
    • 1
  • Hyun Kang
    • 1
    Email author
  1. 1.Department of BiotechnologyKonkuk UniversityChungjuKorea
  2. 2.Department of Biological SciencesKonkuk UniversitySeoulKorea

Personalised recommendations