Novel Fungal Pelletization-Assisted Technology for Algae Harvesting and Wastewater Treatment
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A novel fungi pelletization-assisted bioflocculation technology was developed for efficient algae harvesting and wastewater treatment. Microalga Chlorella vulgaris UMN235 and two locally isolated fungal species Aspergillus sp. UMN F01 and UMN F02 were used to study the effect of various cultural conditions on pelletization process for fungi–algae complex. The results showed that pH was the key factor affecting formation of fungi–algae pellet, and pH could be controlled by adjusting glucose concentration and fungal spore number added. The best pelletization happened when adding 20 g/L glucose and approximately 1.2E8/L spores in BG-11 medium, under which almost 100 % of algal cells were captured onto the pellets with shorter retention time. The fungi–algae pellets can be easily harvested by simple filtration due to its large size (2–5 mm). The filtered fungi–algae pellets were reused as immobilized cells for treatment wastewaters and the nutrient removal rates of 100, 58.85, 89.83, and 62.53 % (for centrate) and 23.23, 44.68, 84.70, and 70.34 % (for diluted swine manure wastewater) for ammonium, total nitrogen, total phosphorus, and chemical oxygen demand, respectively, under both 1- and 2-day cultivations. The novel technology developed is highly promising compared with current algae harvesting and biological wastewater treatment technologies in the literature.
KeywordsFungi pelletization Microalgae algae harvesting Municipal wastewater Animal wastewater Wastewater treatment
The study was supported in part by grants from the University of Minnesota Initiative for Renewable Energy and the Environment and Metropolitan Council Environmental Services, as well as the Legislative-Citizen Commission on Minnesota Resource, and the MOE Biomass Engineering Center in China.
- 5.Gudin, C., & Therpenier, C. (1986). Bioconversion of solar energy into organic chemicals by microalgae. Advances in Biotechnological Processes, 6, 73–110.Google Scholar
- 6.Li, Y., Horsman, M., Wu, N., Lan, C., & Dubois-Calero, N. (2008). Biofuels from microalgae. Biotechnology Progress, 24(4), 815–20.Google Scholar
- 8.Salim, S., Bosma, R., Vermuë, M. H., & Wijffels, R. H. (2010). Harvesting of microalgae by bio-flocculation. Journal of Applied Phycology. doi: 10.1007/s10811-010-9591-x.
- 9.Li, Y., Horsman, M., Wu, N., Lan, C. Q., & Dubois-Calero, N. (2008). Biofuels from microalgae. Biotechnology Progress, 24(4), 815–20.Google Scholar
- 12.Benemann and Oswald (1996)Google Scholar
- 14.Liao, W., Liu, Y., Frear, C., & Chen, S. (2007). A new approach of pellet formation of a filamentous fungus-Rhizopus oryzae. Bioresource Technology, 98, 3415–3423Google Scholar
- 16.Nielsen, J., & Carlsen, M. (1996). Fungal pellets. In R. G. Willaert, G. V. Baron, & L. De Backer (Eds.), Immobilised living cell systems (pp. 273–293). New York: Wiley.Google Scholar
- 22.Van Etten, J. L., Lane, L. C., & Meints, R. H. (1991). Viruses and viruslike particles of eukaryotic algae. Microbiological Reviews, 55(4), 586–620.Google Scholar
- 23.Li, Y., Zhou, W., Hu, B., Min, M., Chen, P., & Ruan, R. (2011). Integration of algae cultivation as biodiesel production feedstock with municipal wastewater treatment: Strains screening and significance evaluation of environmental factors. Bioresource Technology. doi: 10.1016/j.biortech.2011.09.064.
- 24.Wang, L., Min, M., Li, Y., Chen, P., Chen, Y., Liu, Y., Wang, Y., & Ruan, R. (2009). Cultivation of green algae Chlorella sp. in different wastewaters from municipal wastewater treatment plant. Applied Biochemistry and Biotechnology. doi: 10.1007/s12010-009-8866-7.
- 25.Tam, N. F. Y., Lau, P. S., & Wong, Y. S. (1994). Wastewater inorganic N and P removal by immobilized Chlorella vulgaris. Water Science and Technology, 30, 369–374.Google Scholar
- 32.Hach. (2008). Procedure manual. Loveland: Hach.Google Scholar
- 35.Foster, J. W. (1949). Chemical activities of fungi. New York: Academic.Google Scholar
- 37.Kotzabasis, K., Hatziathanasiou, A., Bengoa-Ruigomez, M. V., Kentouri, M., & Divanach, P. (1999). Methanol as alternative carbon source for quicker efficient production of the microalgae Chlorella minutissima: role of the concentration and frequency of administration. Journal of Biotechnology, 70, 357–362.CrossRefGoogle Scholar
- 38.Endo, H., Hosoya, H., & Koibuchi, T. (1977). Growth yield of Chlorella regularis in dark-heterotrophic continuous culture using acetate. Journal of Fermentation Technology, 55, 369–379.Google Scholar
- 39.Lusk, P. (1998). Methane recovery from animal manures the current opportunities casebook. National Renewable Energy Laboratory, NREL/SR-580-25145, September.Google Scholar
- 40.Cheng, J., & Liu, B. (2002). Swine wastewater treatment in anaerobic digesters with floating medium. Transactions of ASAE, 45, 799–805.Google Scholar
- 43.Reeves, T. (1972). Nitrogen removal: a literature review. Journal of the Water Pollution Control Federation, 44, 1895.Google Scholar
- 44.Matusiak, K., Pryztocka-Jusiak, M., Leszczynska-Gerula, K., & Horoch, M. (1976). Studies on the purification of wastewater from the nitrogen fertilizer industry by intensive algal cultures. II: Removal of nitrogen from wastewater. Acta Microbiologica Polonica, 25, 361–74.Google Scholar
- 48.Xin, B. X., Xia, Y. T., Zhang, Y., Aslan, H., Liu, C. H., & Chen, S. (2012). A feasible method for growing fungal pellets in a column reactor inoculated with mycelium fragments and their application for dye bioaccumulation from aqueous solution. Bioresource Technology, 105, 100–105.CrossRefGoogle Scholar