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Characteristics of the Toxin Extracted from Liquid Culture of Colletotrichum capsici F. nicotianae

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Abstract

Colletotrichum capsici f. nicotianae is an important plant pathogen in tobacco-grown area of Weifang region of Shangdong Province, China. In this study, the toxicity of liquid culture media from different isolates was characterized, and some properties of the toxic ingredient were identified. The results indicated that the optimal toxin-producing conditions for C. capsici f. nicotianae were in potato dextrose broth under pH 6.0, at 25∼30 °C for 13 days. The liquid culture media from all isolates were toxic to tobacco plants and induced the wilting symptoms. The toxin from the liquid culture media has thermal, acid–base stability and a broad spectrum of toxicity to the plants. Furthermore, the direct bioassay for two components of the liquid filtrates precipitated by ethanol showed that the active ingredient of the toxin is a kind of nonprotein substance, which was further supported by the papain hydrolysis test.

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Authors and Affiliations

  1. Department of Plant Pathology, Shandong Agricultural University, Taian, 271018, China

    Guang-min Zhang & Bao-hai Fang

  2. Shanghai Information Center for Life Sciences, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200031, China

    Heng Chen

  3. College of Life Science & Biotechnology, Shanghai JiaoTong University, Shanghai, 200030, China

    Xue-ling Li

Authors
  1. Guang-min Zhang
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  2. Bao-hai Fang
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  3. Heng Chen
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  4. Xue-ling Li
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Correspondence to Heng Chen or Xue-ling Li.

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Zhang, Gm., Fang, Bh., Chen, H. et al. Characteristics of the Toxin Extracted from Liquid Culture of Colletotrichum capsici F. nicotianae . Appl Biochem Biotechnol 167, 52–61 (2012). https://doi.org/10.1007/s12010-012-9656-1

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  • DOI: https://doi.org/10.1007/s12010-012-9656-1

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