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Initial Proteome Analysis of Caffeine-Induced Proteins in Aspergillus tamarii Using Two-Dimensional Fluorescence Difference Gel Electrophoresis

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Abstract

Caffeine is toxic to most microorganisms. However, some filamentous fungi, such as Aspergillus tamarii, are able to metabolize this alkaloid when fed caffeine as the sole nitrogen source. The aim of the present work was to identify intracellular A. tamarii proteins, regulated by caffeine, using fluorescence difference two-dimensional gel electrophoresis. Specific proteins from two culture media of A. tamarii grown either on ammonium sulfate or caffeine as the sole nitrogen source were analysed by mass spectrometry. Thirteen out of a total of 85 differentially expressed spots were identified after database search. Identified up-regulated proteins include phosphoglycerate kinase, malate dehydrogenase, dyp-type peroxidase family protein, heat shock protein, Cu, Zn superoxidase dismutase and xanthine dehydrogenase. Some of the proteins identified in this study are involved in the caffeine degradation pathway as well as in stress response, suggesting that stress proteins could be involved in caffeine metabolism in filamentous fungi.

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Acknowledgements

We gratefully acknowledge Dr. Tracy Andacht and Dr. Maria E. for the technical advice. Present work was supported by the Institut de Recherche pour le Développement (IRD-France). G. Gutiérrez-Sánchez was awarded a Ph.D. scholarship by the National Council of Science and Technology (CONACyT, Mexico), Grant No. 130250.

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Authors and Affiliations

  1. Institut de Recherche pour le Développement, Faculté des Sciences de Saint Jérôme; Case 441, Université Paul Cézanne, Marseille, France

    Gerardo Gutiérrez-Sánchez, Sévastianos Roussos & Christopher Augur

  2. Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA, 30602-4712, USA

    Gerardo Gutiérrez-Sánchez, James Atwood & Christopher Augur

  3. Windber Research Institute, Windber, PA, USA

    V. S. Kumar Kolli

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  1. Gerardo Gutiérrez-Sánchez
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  2. James Atwood
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  3. V. S. Kumar Kolli
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  4. Sévastianos Roussos
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  5. Christopher Augur
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Correspondence to Gerardo Gutiérrez-Sánchez.

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This paper is dedicated to the memory of Christopher Augur, esteemed colleague and friend.

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Gutiérrez-Sánchez, G., Atwood, J., Kolli, V.S.K. et al. Initial Proteome Analysis of Caffeine-Induced Proteins in Aspergillus tamarii Using Two-Dimensional Fluorescence Difference Gel Electrophoresis. Appl Biochem Biotechnol 166, 2064–2077 (2012). https://doi.org/10.1007/s12010-012-9634-7

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  • DOI: https://doi.org/10.1007/s12010-012-9634-7

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