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Induced Biofilm Cultivation Enhances Riboflavin Production by an Intertidally Derived Candida famata

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Abstract

The aim of the investigation was to ascertain if surface attachment of Candida famata and aeration enhanced riboflavin production. A newly designed polymethylmethacrylate (PMMA) conico-cylindrical flask (CCF) holding eight equidistantly spaced rectangular strips mounted radially on a circular disk allowed comparison of riboflavin production between CCFs with hydrophobic surface (PMMA-CCF), hydrophilic glass surface (GS-CCF), and 500-ml Erlenmeyer flask (EF). Riboflavin production (mg/l) increased from 12.79 to 289.96, from 54.44 to 238.14, and from 36.98 to 158.71 in the GS-CCF, EF, and PMMA-CCF, respectively, when C. famata was grown as biofilm-induced cultures in contrast to traditional planktonic culture. Production was correlated with biofilm formation and planktonic growth was suppressed in cultivations that allowed higher biofilm formation. Enhanced aeration increased riboflavin production in hydrophilic vessels. Temporal pattern of biofilm progression based on two-channel fluorescence detection of extracellular polymeric substances and whole cells in a confocal laser scanning microscope followed by application of PHLIP and ImageJ volume viewer software demonstrated early maturity of a well-developed, stable biofilm on glass in contrast to PMMA surface. A strong correlation between hydrophilic reactor surface, aeration, biofilm formation, and riboflavin production was established in C. famata. Biofilm culture is a new-found means to improve riboflavin production by C. famata.

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Acknowledgments

Financial support to JM, RG (DBT sanction no. BT/PR11479/AAQ/03/423/2008), and DST-PURSE, 2009–2010 is thankfully acknowledged. The authors wish to thank Dr. Arun Bandyopadhyay and Mrs. Banasri Das for their help during confocal microscopy. DT wishes to thank the authorities of IIT (Guwahati) for the summer internship.

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Authors and Affiliations

  1. School of Environmental Studies, Jadavpur University, Kolkata, 700 032, India

    Sayani Mitra & Joydeep Mukherjee

  2. Department of Biotechnology, Indian Institute of Technology, Guwahati, 781 039, India

    Dheeraj Thawrani

  3. Division of Cell Biology and Physiology, Indian Institute of Chemical Biology, Jadavpur, Kolkata, 700 032, India

    Priyam Banerjee

  4. Department of Life Science and Biotechnology, Jadavpur University, Kolkata, 700 032, India

    Ratan Gachhui

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  1. Sayani Mitra
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  2. Dheeraj Thawrani
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  3. Priyam Banerjee
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  4. Ratan Gachhui
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  5. Joydeep Mukherjee
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Correspondence to Joydeep Mukherjee.

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Fig. S1

Estimated 3D surface plots of C. famata biofilm following analysis by ImageJ using the interactive 3D surface plot plugin 2.32 after A 1, B 2, C 3, and D 4 days on glass surface and after E 4, F 5, G 6, and H 7 days on the PMMA surface. Color only in online version (JPEG 30 kb)

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Mitra, S., Thawrani, D., Banerjee, P. et al. Induced Biofilm Cultivation Enhances Riboflavin Production by an Intertidally Derived Candida famata . Appl Biochem Biotechnol 166, 1991–2006 (2012). https://doi.org/10.1007/s12010-012-9626-7

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