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Bioethanol Production Involving Recombinant C. thermocellum Hydrolytic Hemicellulase and Fermentative Microbes

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Abstract

The enhancement of the biomass productivity of Escherichia coli cells harbouring the truncated 903 bp gene designated as glycoside hydrolase family 43 (GH43) from Clostridium thermocellum showing hemicellulase activity along with its further use in simultaneous saccharification and fermentation (SSF) process is described. (Phosphoric acid) H3PO4–acetone treatment and ammonia fibre expansion (AFEX) were the pretreatment strategies employed on the leafy biomass of mango, poplar, neem and asoka among various substrates owing to their high hemicellulose content. GH43 showed optimal activity at a temperature of 50 °C, pH 5.4 with stability over a pH range of 5.0–6.2. A 4-fold escalation in growth of the recombinant E. coli cells was observed when grown using repeated batch strategy in LB medium supplemented with glucose as co-substrate. Candida shehatae utilizing pentose sugars was employed for bioethanol production. AFEX pretreatment proved to be better over acid–acetone technique. The maximum ethanol concentration (1.44 g/L) was achieved for AFEX pretreated mango (1%, w/v) followed by poplar with an ethanol titre (1.32 g/L) in shake flask experiments. A 1.5-fold increase in ethanol titre (2.11 g/L) was achieved with mango (1%, w/v) in a SSF process using a table top 2-L bioreactor with 1 L working volume.

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Acknowledgments

The research work and S. P. Das supported by a project grant from Department of Biotechnology, Ministry of Science and Technology, New Delhi, India to AG is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Biotechnology, Indian Institute of Technology Guwahati, Guwahati, 781039, Assam, India

    Saprativ P. Das, Rajeev Ravindran, Shadab Ahmed, Debasish Das & Arun Goyal

  2. Department of Biotechnology and Environmental Sciences, Thapar University, Bhadson Road, Patiala, 140007, Punjab, India

    Dinesh Goyal

  3. CIISA—Faculdade de Medicina Veterinaria, Avenida da Universidade Técnica, 1300 477, Lisbon, Portugal

    Carlos M. G. A. Fontes

Authors
  1. Saprativ P. Das
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  2. Rajeev Ravindran
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  3. Shadab Ahmed
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  4. Debasish Das
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  5. Dinesh Goyal
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  6. Carlos M. G. A. Fontes
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  7. Arun Goyal
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Corresponding author

Correspondence to Arun Goyal.

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Saprativ P. Das, Rajeev Ravindran and Shadab Ahmed contributed equally to this work.

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Das, S.P., Ravindran, R., Ahmed, S. et al. Bioethanol Production Involving Recombinant C. thermocellum Hydrolytic Hemicellulase and Fermentative Microbes. Appl Biochem Biotechnol 167, 1475–1488 (2012). https://doi.org/10.1007/s12010-012-9618-7

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  • DOI: https://doi.org/10.1007/s12010-012-9618-7

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