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Butanol Production from Cane Molasses by Clostridium saccharobutylicum DSM 13864: Batch and Semicontinuous Fermentation

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Abstract

Clostridium acetobutylicum strains used in most Chinese ABE (acetone–butanol–ethanol) plants favorably ferment starchy materials like corn, cassava, etc., rather than sugar materials. This is one major problem of ABE industry in China and significantly limits the exploitation of cheap waste sugar materials. In this work, cane molasses were utilized as substrate in ABE production by Clostridium saccharobutylicum DSM 13864. Under optimum conditions, total solvent of 19.80 g/L (13.40 g/L butanol) was reached after 72 h of fermentation in an Erlenmeyer flask. In a 5-L bioreactor, total solvent of 17.88 g/L was attained after 36 h of fermentation, and the productivity and yield were 0.50 g/L/h and 0.33 g ABE/g sugar consumption, respectively. To further enhance the productivity, a two-stage semicontinuous fermentation process was steadily operated for over 8 days (205 h, 26 cycles) with average productivity (stage II) of 1.05 g/L/h and cell concentration (stage I) of 7.43 OD660, respectively. The average batch fermentation time (stage I and II) was reduced to 21−25 h with average solvent of 15.27 g/L. This study provides valuable process data for the development of industrial ABE fermentation process using cane molasses as substrate.

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Acknowledgments

The authors gratefully acknowledge the support of New Century Excellent Talents in University (NCET-11-0658), the Program of Introducing Talents of Discipline to Universities No. 111-2-06, the Priority Academic Program Development of Jiangsu Higher Education Institutions, and Green Biologics Ltd (Oxfordshire, UK).

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Laboratory of Biocatalysis, School of Biotechnology, Jiangnan University, 1800 Lihu Rd., Wuxi, 214122, People’s Republic of China

    Ye Ni, Yun Wang & Zhihao Sun

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  1. Ye Ni
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  2. Yun Wang
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  3. Zhihao Sun
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Corresponding author

Correspondence to Ye Ni.

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Fig. A1

The effect of pH (a), trace element (b), nitrogen sources (c), and content of calcium carbonate (d) on ABE fermentation by C. saccharobutylicum DSM 13864. The fermentation medium and conditions were as follows: (a) 6% cane molasses, 3.2 g/L CaCO3, 2 g/L (NH4)2SO4, 0.5 g/L K2HPO4, pH 5.6−8.0, 35 °C; (b) 6% cane molasses, 3.2 g/L CaCO3, 2 g/L (NH4)2SO4, 0.5 g/L K2HPO4, 0.01 g/L trace element, pH 6.0, 35 °C; (c) 6% cane molasses, 3.2 g/L CaCO3, 2 g/L inorganic nitrogen sources or 2 g/L (NH4)2SO4, and 10 g/L organic nitrogen sources, 0.5 g/L K2HPO4, pH 6.0, 35 °C; (d) 6% cane molasses, 2.4−4.0 g/L CaCO3, 2 g/L (NH4)2SO4, 0.5 g/L K2HPO4, 20 g/L CSP, 0.01 g/L MnSO4·H2O, pH 6.0, 37 °C (JPEG 9 kb)

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Ni, Y., Wang, Y. & Sun, Z. Butanol Production from Cane Molasses by Clostridium saccharobutylicum DSM 13864: Batch and Semicontinuous Fermentation. Appl Biochem Biotechnol 166, 1896–1907 (2012). https://doi.org/10.1007/s12010-012-9614-y

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  • DOI: https://doi.org/10.1007/s12010-012-9614-y

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