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Laccase Production by the Aquatic Ascomycete Phoma sp. UHH 5-1-03 and the White Rot Basidiomycete Pleurotus ostreatus DSM 1833 During Submerged Cultivation on Banana Peels and Enzyme Applicability for the Removal of Endocrine-Disrupting Chemicals

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Abstract

This work aimed to study the production of laccase from Pleurotus ostreatus DSM 1833 and Phoma sp. UHH 5-1-03 using banana peels as alternative carbon source, the subsequent partial purification and characterization of the enzyme, as well the applicability to degrade endocrine disruptors. The laccase stability with pH and temperature, the optimum pH, the K m and V max parameters, and the molar mass were determined. Tests were conducted for assessing the ability of degradation of the endocrine disruptors t-nonylphenol, bisphenol A, and 17α-ethinylestradiol. Laccase production of 752 and 1,117 U L−1 was obtained for Phoma sp. and P. ostreatus, respectively. Phoma sp. laccase showed higher stability with temperature and pH. The laccase from both species showed higher affinity by syringaldazine. The culture broth with banana peels induced the production of two isoforms of P. ostreatus (58.7 and 21 kDa) and one of Phoma sp. laccase (72 kDa). In the first day of incubation, the concentrations of bisphenol A and 17α-ethinylestradiol were reduced to values close to zero and after 3 days the concentration of t-nonylphenol was reduced in 90% by the P. ostreatus laccase, but there was no reduction in its concentration by the Phoma sp. laccase.

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Acknowledgments

We gratefully acknowledge Dr. Rainer Jonas for the support during the internship in Germany as well as InWent-International Capacity Building. We also acknowledge the Santa Catarina State Research Foundation—FAPESC and the Coordination for the Improvement of Higher Level Education Personnel—CAPES for the financial support. Furthermore, we want to thank R. Boetz (UFZ) for the excellent technical assistance.

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Authors and Affiliations

  1. UFZ, Department of Environmental Microbiology, UFZ, Helmholz Centre for Environmental Research, Permoserstrasse 15, 04318, Leipzig, Germany

    Nelson Libardi Junior & Dietmar Schlosser

  2. Department of Chemical Engineering, UNIVILLE, University of the Region of Joinville, M Box 246, CEP 89.201-972, Joinville, Brazil

    Nelson Libardi Junior, Regina Maria Miranda Gern & Sandra Aparecida Furlan

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  1. Nelson Libardi Junior
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  2. Regina Maria Miranda Gern
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  3. Sandra Aparecida Furlan
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  4. Dietmar Schlosser
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Correspondence to Regina Maria Miranda Gern.

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Libardi Junior, N., Gern, R.M.M., Furlan, S.A. et al. Laccase Production by the Aquatic Ascomycete Phoma sp. UHH 5-1-03 and the White Rot Basidiomycete Pleurotus ostreatus DSM 1833 During Submerged Cultivation on Banana Peels and Enzyme Applicability for the Removal of Endocrine-Disrupting Chemicals. Appl Biochem Biotechnol 167, 1144–1156 (2012). https://doi.org/10.1007/s12010-012-9601-3

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