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Isolation and Characterization of Zygomycetes Fungi from Tempe for Ethanol Production and Biomass Applications

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Abstract

Mixed fungal cultures used for making tempe, a fermented soy bean food, were screened for biomass conversion. Thirty-two zygomycetes strains from two tempe cultures were isolated and identified as Rhizopus, Mucor, Rhizomucor, and Absidia species based upon morphology. The dry weight biomass of these strains contained 49% to 63% protein and 10–24% chitosan. The strains with the best growth performance were selected and registered at Culture Collection of Gothenburg University as Rhizomucor CCUG 61146 and Rhizomucor CCUG 61147. These strains were able to grow both aerobically and micro-aerobically. Their ethanol yields were 0.38–0.47, 0.19–0.22, and 0.31–0.38 g/g on glucose, xylose, and a mix sugars consisting of cellobiose, glucose, xylose, arabinose, galactose, and mannose, respectively. The biomass yield of the strains varied between 65 and 140 mg dry weight/g glucose.

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References

  1. REN21. (2011). Renewables 2011 global status report (1st ed.). Paris: REN21 Secretariat.

    Google Scholar 

  2. Wyman, C. E., Dale, B. E., Elander, R. T., Holtzapple, M., Ladisch, M. R., & Lee, Y. Y. (2005). Bioresource Technology, 96, 1959–1966.

    Article  CAS  Google Scholar 

  3. Taherzadeh, M. J., & Karimi, K. (2008). International Journal of Molecular Sciences, 9, 1621–1651.

    Article  CAS  Google Scholar 

  4. Millati, R., Edebo, L., & Taherzadeh, M. J. (2005). Enzyme and Microbial Technology, 36, 294–300.

    Article  CAS  Google Scholar 

  5. Lee, J. (1997). Journal of Biotechnology, 56, 1–24.

    Article  CAS  Google Scholar 

  6. Chiang, C., & Knight, S. G. (1960). Biochemical and Biophysical Research Communications, 3, 554–559.

    Article  CAS  Google Scholar 

  7. Verho, R., Putkonen, M., Londesborough, J., Penttilä, M., & Richard, P. (2004). Journal of Biological Chemistry, 279, 14746–14751.

    Article  CAS  Google Scholar 

  8. Rizzi, M., Erlemann, P., Bui-Thanh, N.-A., & Dellweg, H. (1988). Applied Microbiology and Biotechnology, 29, 148–154.

    Article  CAS  Google Scholar 

  9. Carlile, M. J. (1995). In N. A. R. Gow & G. M. Gadd (Eds.), The growing fungus (pp. 3–19). London: Chapman & Hall.

    Google Scholar 

  10. Dijksterhuis, J., & Samson, R. A. (2006). In C. W. Blackburn (Ed.), Food spoilage microorganisms (pp. 415–436). Cambridge: Woodhead Publishing Limited.

    Chapter  Google Scholar 

  11. Edebo, L. (2009). Zygomycetes for fish feed. US Patent 2009136617

  12. Tan, S. C., Tan, T. K., Wong, S. M., & Khor, E. (1996). Carbohydrate Polymers, 30, 239–242.

    Article  CAS  Google Scholar 

  13. White, S. A., Farina, P. R., & Fulton, I. (1979). Applied and Environmental Microbiology, 38, 323–328.

    CAS  Google Scholar 

  14. Davoust, N., & Hansson, G. (1992). Applied Microbiology and Biotechnology, 36, 618–620.

    Article  CAS  Google Scholar 

  15. Hu, K.-J., Hu, J.-L., Ho, K.-P., & Yeung, K.-W. (2004). Carbohydrate Polymers, 58, 45–52.

    Article  CAS  Google Scholar 

  16. Steinkraus, K. H. (2004). Industrialization of indegenous fermented foods (2nd ed.). New York: Marcel Dekker.

    Book  Google Scholar 

  17. Samson, R. A., Hoekstra, E. S., Frisvad, J. C., & Filtenborg, O. (1995). Introduction to food-borne fungi (4th ed.). Baarn: Centraalbureau voor Schimmelcultures.

    Google Scholar 

  18. Zamani, A., Jeihanipour, A., Edebo, L., Niklasson, C., & Taherzadeh, M. J. (2008). Journal of Agricultural and Food Chemistry, 56, 8314–8318.

    Article  CAS  Google Scholar 

  19. Synowiecki, J., & AlKhateeb, N. A. A. Q. (1997). Food Chemistry, 60, 605–610.

    Article  CAS  Google Scholar 

  20. Zamani, A., Edebo, L., Sjostrom, B., & Taherzadeh, M. J. (2007). Biomacromolecules, 8, 3786–3790. Epub 2007 Nov 3727.

    Article  CAS  Google Scholar 

  21. Zamani, A., Edebo, L., Niklasson, C., & Taherzadeh, M. J. (2010). International Journal of Molecular Sciences, 11, 2976–2987.

    Article  CAS  Google Scholar 

  22. Verduyn, C., Postma, E., Scheffers, W. A., & Van Dijken, J. P. (1990). Journal of General Microbiology, 136, 395–403.

    Article  CAS  Google Scholar 

  23. Buyukkileci, A. G. (2007). Ph.D. thesis, Middle East Technical University, Ankara.

  24. Dutta, P. K., Tripathi, S., Mehrotra, G. K., & Dutta, J. (2009). Food Chemistry, 114, 1173–1182.

    Article  CAS  Google Scholar 

  25. Tharanathan, R. N., & Kittur, F. S. (2003). Critical Reviews in Food Science and Nutrition, 43, 61.

    Article  CAS  Google Scholar 

  26. Batt, C. A., Carvallo, S., Easson, D. D., Akedo, M., & Sinskey, A. J. (1986). Biotechnology and Bioengineering, 28, 549–553.

    Article  CAS  Google Scholar 

  27. Senac, T., & Hahn-Hägerdal, B. (1990). Applied and Environmental Microbiology, 56, 120–126.

    CAS  Google Scholar 

  28. Wu, J. F., Lastick, S. M., & Updegraff, D. M. (1986). Nature, 321, 887–888.

    Article  CAS  Google Scholar 

  29. Skory, C. D., Freer, S. N., & Bothast, R. J. (1997). Biotechnology Letters, 19, 203–206.

    Article  CAS  Google Scholar 

  30. Sues, A., Millati, R., Edebo, L., & Taherzadeh, M. J. (2005). FEMS Yeast Research, 5, 669–676.

    Article  CAS  Google Scholar 

  31. Aranaz, I., Mengibar, M., Harris, R., Panos, I., Miralles, B., Acosta, N., et al. (2009). Current Chemical Biology, 3, 203–230.

    Article  CAS  Google Scholar 

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Acknowledgments

This work was financially supported by the European Erasmus Mundus External Cooperation Windows (EMECW) program and the Swedish Energy Agency.

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Authors and Affiliations

  1. School of Engineering, University of Borås, Borås, Sweden

    Rachma Wikandari, Patrik R. Lennartsson & Mohammad J. Taherzadeh

  2. Study Program of Biotechnology, Gadjah Mada University, Yogyakarta, Indonesia

    Rachma Wikandari

  3. Food and Agricultural Product Technology Department, Gadjah Mada University, Yogyakarta, Indonesia

    Ria Millati & Eni Harmayani

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  1. Rachma Wikandari
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  2. Ria Millati
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  3. Patrik R. Lennartsson
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  4. Eni Harmayani
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  5. Mohammad J. Taherzadeh
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Correspondence to Mohammad J. Taherzadeh.

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Wikandari, R., Millati, R., Lennartsson, P.R. et al. Isolation and Characterization of Zygomycetes Fungi from Tempe for Ethanol Production and Biomass Applications. Appl Biochem Biotechnol 167, 1501–1512 (2012). https://doi.org/10.1007/s12010-012-9587-x

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  • DOI: https://doi.org/10.1007/s12010-012-9587-x

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