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Efficient Microbial Conversion of l-Tyrosine to l-DOPA by Brevundimonas sp. SGJ

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Abstract

l-DOPA (3,4-dihydroxyphenyl-l-alanine), the most widely used drug for the treatment of Parkinson’s disease, was produced in buffer using biomass of Brevundimonas sp. SGJ. The effects of enhancers, such as carrageenan, diatomaceous earth, and activated charcoal, on the l-DOPA production were evaluated to obtain the maximum yield. The optimal process conditions found were pH 8, 2 g l−1 cell mass, 2 g l−1 l-tyrosine, 0.04 g l−1 CuSO4, 0.02 g l−1 l-ascorbic acid, 0.5 g l−1 carrageenan, and 40 °C temperature. In addition, repeated use of cells resulted in the highest yield of 3.81 g l−1 (95.2%) of l-DOPA with utilization of 4 g l−1 l-tyrosine, and the highest tyrosinase activity (9,201 U mg−1) was observed at 18 h of incubation. Furthermore, the produced l-DOPA was confirmed by high-performance thin-layer chromatography, high-performance liquid chromatography, and gas chromatography–mass spectroscopy. Kinetic studies showed significant values of Y p/s, Q s, and q s after optimization of the process. Thus, Brevundimonas sp. SGJ could be an eventual new source for large-scale production of l-DOPA.

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Acknowledgment

Shripad N. Surwase, one of the authors, is thankful to Lady Tata Memorial Trust, Mumbai, Maharashtra, India for awarding Junior Research Scholarship for his doctoral research in 2010–2011.

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Authors and Affiliations

  1. Department of Microbiology, Shivaji University, Kolhapur, 416004, India

    Shripad N. Surwase

  2. Department of Biotechnology, Shivaji University, Vidyanagar, Kolhapur, 416004, India

    Sushama A. Patil, Onkar A. Apine & Jyoti P. Jadhav

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  1. Shripad N. Surwase
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  2. Sushama A. Patil
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  3. Onkar A. Apine
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  4. Jyoti P. Jadhav
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Correspondence to Jyoti P. Jadhav.

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Surwase, S.N., Patil, S.A., Apine, O.A. et al. Efficient Microbial Conversion of l-Tyrosine to l-DOPA by Brevundimonas sp. SGJ. Appl Biochem Biotechnol 167, 1015–1028 (2012). https://doi.org/10.1007/s12010-012-9564-4

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  • DOI: https://doi.org/10.1007/s12010-012-9564-4

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