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Crystalline Xylitol Production by a Novel Yeast, Pichia caribbica (HQ222812), and Its Application for Quorum Sensing Inhibition in Gram-Negative Marker Strain Chromobacterium violaceum CV026

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Abstract

Xylitol, a sugar alcohol, is fast gaining ground over other artificial sugar substitutes owing to its advantageous properties. Xylitol is a safer alternative for diabetics because of insulin-independent metabolism. It has beneficial properties suitable to form an important part of odontological formulations. Conventional commercial production of xylitol involves harsh chemical method operating at high temperature and pressure. Thus, microbial production of xylitol is preferred over chemical method, and yeasts have been extensively exploited for this purpose. In the present manuscript, quantitative production of xylitol from d-xylose with the yield of 0.852 gm/gm and volumetric productivity of 1.83 gm/l/h in crystalline form, using novel yeast Pichia caribbica is reported. Also, a mild, safe procedure for product extraction is described. The ability of xylitol to act as a quorum sensing antagonist in gram-negative marker strain Chromobacterium violaceum CV026 has been demonstrated for the first time.

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Acknowledgments

RM and KJ-N are extremely grateful to University Grants Commission for the Research fellowship. RM wishes to thank Dr. Paul Williams, University of Nottingham, UK, for kindly providing the bioassay culture CV026 as a gift. Also, we wish to thank Dr. Nandini Devi, National Chemical Laboratory, Pune, for her timely help with XRD data analysis.

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Authors and Affiliations

  1. Division of Biochemical Sciences, National Chemical Laboratory, Homi Bhabha road, Pune, 411008, Maharashtra, India

    Ruchira Mukherji, Kasturi Joshi-Navare & Asmita Prabhune

Authors
  1. Ruchira Mukherji
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  2. Kasturi Joshi-Navare
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  3. Asmita Prabhune
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Corresponding author

Correspondence to Asmita Prabhune.

Additional information

International patent has been filed based on the present work (provisional number-2190DEL2012). And the culture has been deposited as per Budapest Treaty IDA guidelines (culture accession number-MTCC-5703)

The authors Ruchira Mukherji and Kasturi Joshi-Navare have contributed equally to this work.

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Mukherji, R., Joshi-Navare, K. & Prabhune, A. Crystalline Xylitol Production by a Novel Yeast, Pichia caribbica (HQ222812), and Its Application for Quorum Sensing Inhibition in Gram-Negative Marker Strain Chromobacterium violaceum CV026. Appl Biochem Biotechnol 169, 1753–1763 (2013). https://doi.org/10.1007/s12010-012-0039-4

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  • DOI: https://doi.org/10.1007/s12010-012-0039-4

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