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Indole-3-Acetic Acid Biosynthesis in Fusarium delphinoides Strain GPK, a Causal Agent of Wilt in Chickpea

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Abstract

Fusarium delphinoides (Ascomycota; Nectriaceae) is an indole-3-acetic acid (IAA) producing plant pathogen and a causal agent of wilt in chickpea. The IAA biosynthetic pathway in F. delphinoides strain GPK (FDG) was examined by analyzing metabolic intermediates and by feeding experiments. Gas chromatograph (GC) analysis of FDG culture filtrates showed the presence of metabolic intermediates of indole-3-pyruvic acid (IPyA), indole-3-acetamide (IAM), and tryptamine (TRA) pathways. The different IAA biosynthetic pathways were further confirmed by identifying the presence of different enzymes of these pathways. Substrate specificity study of aromatic amino acid aminotransferase revealed that the enzyme is highly specific for tryptophan (Trp) and α-ketoglutarate (α-kg) as amino group donor and acceptor, respectively. Furthermore, the concentration-dependent effect of exogenous IAA on fungal growth was established. Low concentration of exogenous IAA increases the fungal growth and at high concentration it decreases the growth of FDG.

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Acknowledgments

Authors wish to thank University Grants Commission (UGC), New Delhi, India for the financial support through Major Research Project and SAP programme. One of the author Guruprasad B. Kulkarni wishes to thank Council of Scientific and Industrial Research (CSIR) for providing financial assistance through Senior Research Fellowship (SRF).

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Authors and Affiliations

  1. Department of Biochemistry, Gulbarga University, Gulbarga, 585106, Karnataka, India

    Guruprasad B. Kulkarni, S Sanjeevkumar, B. Kirankumar, M. Santoshkumar & T. B. Karegoudar

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  1. Guruprasad B. Kulkarni
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  2. S Sanjeevkumar
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  3. B. Kirankumar
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  4. M. Santoshkumar
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  5. T. B. Karegoudar
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Correspondence to T. B. Karegoudar.

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Kulkarni, G.B., Sanjeevkumar, S., Kirankumar, B. et al. Indole-3-Acetic Acid Biosynthesis in Fusarium delphinoides Strain GPK, a Causal Agent of Wilt in Chickpea. Appl Biochem Biotechnol 169, 1292–1305 (2013). https://doi.org/10.1007/s12010-012-0037-6

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  • DOI: https://doi.org/10.1007/s12010-012-0037-6

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