Abstract
Xylitol, a naturally occurring five-carbon sugar alcohol derived from d-xylose, is currently in high demand by industries. Trichoderma reesei, a prolific industrial cellulase and hemicellulase producing fungus, is able to selectively use d-xylose from hemicelluloses for xylitol production. The xylitol production by T. reesei can be enhanced by genetic engineering of blocking further xylitol metabolism in the d-xylose pathway. We have used two different T. reesei strains which are impaired in the further metabolism of xylitol including a single mutant in which the xylitol dehydrogenase gene was deleted (∆xdh1) and a double mutant where additionally l-arabinitol-4-dehydrogenase, an enzyme which can partially compensate for xylitol dehydrogenase function, was deleted (∆lad1∆xdh1). Barely straw was first pretreated using NaOH and Organosolv pretreatment methods. The highest xylitol production of 6.1 and 13.22 g/L was obtained using medium supplemented with 2 % Organosolv-pretreated barley straw and 2 % d-xylose by the ∆xdh1 and ∆lad1∆xdh1 strains, respectively.
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Abbreviations
- ∆xdh1 :
-
Xylitol dehydrogenase gene deleted strain (T. reesei single mutant strain)
- ∆lad1∆xdh1 :
-
l-Arabinitol-4-dehydrogenase and xylitol dehydrogenase genes deleted strain (T. reesei double mutant strain)
- PPP:
-
Pentose phosphate pathway
- FTIR:
-
Fourier transform infrared spectroscopic
- HPAE-PAD:
-
High-performance anion exchange chromatography with pulsed amperometric detection
- DNS:
-
3,5-Dinitrosalicylic acid
- FPU:
-
Filter paper unit
- MCC:
-
Microcrystalline cellulose
- RS:
-
Reducing sugars
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Acknowledgments
The authors would like to thank Dr. Shuna Cheng and Dr. Chunbao (Charles) Xu for their great help with the Organosolv pretreatment. The authors would also like to thank Dr. Matthew Leitch for allowing us to use Wiley mill machine. This work was supported by a scholarship from Ontario Graduate Scholarship to M.D. and NSERC-RCD and Ontario Research Chair funding to W.Q.
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The authors declare that they have no competing interests.
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Dashtban, M., Kepka, G., Seiboth, B. et al. Xylitol Production by Genetically Engineered Trichoderma reesei Strains Using Barley Straw as Feedstock. Appl Biochem Biotechnol 169, 554–569 (2013). https://doi.org/10.1007/s12010-012-0008-y
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DOI: https://doi.org/10.1007/s12010-012-0008-y