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Applied Biochemistry and Biotechnology

, Volume 166, Issue 4, pp 974–986 | Cite as

Microbial Production of Propionic Acid with Propionibacterium freudenreichii Using an Anion Exchanger-Based In Situ Product Recovery (ISPR) Process with Direct and Indirect Contact of Cells

  • Peng Wang
  • Yunshan WangEmail author
  • Zhiguo Su
Article

Abstract

The recovery of an inhibiting product from a bioreactor soon after its formation is an important issue in industrial bioprocess development. In the present study, the potential of the anion exchanger-based in situ product recovery (ISPR) technique for the biocatalytic production of propionic acid was discussed. The focus of the current work was the selection of a suitable configuration of metabolically active cells for application in propionic acid production. Accumulation of propionic acid in fermentation broth caused feedback inhibition of the growth and biotransformation activity of Propionibacterium freudenreichii CICC 10019. Relevant product inhibition kinetics was discussed, and the results showed that keeping the aqueous propionic acid concentration below 10.02 g L−1 was an essential prerequisite for ISPR process. A batch study, in which three ISPR configuration mode designs were compared, was conducted. The comparison indicated that employing an external direct mode had significant advantages over other modes in terms of increased productivity and product yield, with a corresponding decrease in the number of downstream processing steps, as well as in substrate consumption. The fed-batch culture using an external direct mode for the continuous accumulation of propionic acid resulted in a cumulative propionic acid concentration of 62.5 g L−1, with a corresponding product yield of 0.78 g propionic acid/g glucose.

Keywords

Propionibacterium freudenreichii Propionic acid ISPR External direct mode Fed-batch fermentation 

Notes

Acknowledgments

The present work was supported by the Knowledge Innovation Program of the Chinese Academy of Sciences, Grant NO.KSCX2-YW-G-021.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  1. 1.National Key Laboratory of Biochemical Engineering, Institute of Process EngineeringChinese Academy of SciencesBeijingPeople’s Republic of China
  2. 2.Graduate University of the Chinese Academy of SciencesBeijingChina

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