Skip to main content
SpringerLink
Log in

A Thermophilic Cellulase Complex from Phialophora sp. G5 Showing High Capacity in Cellulose Hydrolysis

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A cellulase-producing mesophilic fungal strain, named G5, was isolated from the acidic wastewater and mud of a tin mine and identified as Phialophora sp. based on the internal transcribed spacer sequence. The volumetric activities and specific activities of cellulase induced by different carbon sources (Avicel, corn cob, wheat bran and corn stover) were compared. The cellulase complex of Phialophora sp. G5 exhibited the optimal activities at 60–65 °C and pH 4.0–5.0, and had good long-term thermostability at 50 °C. Compared with the commercial cellulase (Accellerase 1500, Genencor), the enzyme under study showed 60% and 80% of the capacity to hydrolyze pure cellulose and natural cellulose, respectively. This is the first study to report that a cellulytic enzymes complex from Phialophora genus, and the superior properties of this enzyme complex make strain G5 a potential microbial source to produce cellulase for industrial applications, and the production ability could be improved by mutagenesis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Lynd, L. R., Weimer, P. J., van Zyl, W. H., & Pretorius, I. S. (2002). Microbiology and Molecular Biology Reviews, 66, 506–577.

    Article  CAS  Google Scholar 

  2. Ng, I. S., Li, C. W., Chan, S. P., Chir, J. L., Chen, P. T., Tong, C. G., Yu, S. M., & Ho, T. H. D. (2010). Bioresource Technology, 10, 1310–1317.

    Article  Google Scholar 

  3. Henrissat, B. (1994). Cellulose, 1, 169–196.

    Article  CAS  Google Scholar 

  4. Schmoll, M., & Kubicek, C. P. (2003). Acta Microbiologica Immunologica Hungarcia, 50, 125–145.

    Article  CAS  Google Scholar 

  5. Zhang, Y.-H. P., & Lynd, L. R. (2004). Biotechnolology and Bioengineering, 88, 797–824.

    Article  CAS  Google Scholar 

  6. Samayam, I. P., & Schall, C. A. (2010). Bioresource Technology, 101, 3561–3566.

    Article  CAS  Google Scholar 

  7. Shi, Q. Q., Sun, J., Yu, H. L., Li, C. X., Bao, J., & Xu, H. J. (2011). Applied Biochemistry and Biotechnology, 164, 819–830.

    Google Scholar 

  8. Cheng, Y., Song, X., Qin, Y., & Qu, Y. (2009). Journal of Applied Microbiology, 107, 1837–1846.

    Article  CAS  Google Scholar 

  9. Dashtban, M., Schraft, H., & Qin, W. S. (2009). International Journal of Biological Sciences, 5, 578–595.

    Article  CAS  Google Scholar 

  10. Latifian, M., Esfahani, Z. H., & Barzegar, M. (2007). Bioresource Technology, 98, 3634–3637.

    Article  CAS  Google Scholar 

  11. Zhang, Y.-H. P., Himmel, M. E., & Mielenz, J. R. (2006). Biotechnology Advances, 24, 452–481.

    Article  CAS  Google Scholar 

  12. Saitou, N., & Nei, M. (1987). Molecular Biology and Evolution, 4, 406–425.

    CAS  Google Scholar 

  13. Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2007). Molecular Biology and Evolution, 24, 1596–1599.

    Article  CAS  Google Scholar 

  14. Gao, J. M., Weng, H. B., Zhu, D. H., Yuan, M. X., Guan, F. X., & Xi, Y. (2008). Bioresource Technology, 99, 7623–7629.

    Article  CAS  Google Scholar 

  15. Ghose, T. K. (1987). Pure and Applied Chemistry, 59, 257–268.

    Article  CAS  Google Scholar 

  16. Miller, G. L. (1959). Analytical Chemistry, 31, 426–428.

    Article  CAS  Google Scholar 

  17. Valášková, V., & Baldrian, P. (2006). Microbiology, 152, 3613–3622.

    Article  Google Scholar 

  18. Bradford, M. M. (1976). Analytical Biochemistry, 72, 248–254.

    Article  CAS  Google Scholar 

  19. Osono, T., Fukasawa, Y., & Takeda, H. (2003). Mycologia, 95, 820–825.

    Article  Google Scholar 

  20. Voutilainen, S. P., Murray, P. G., Tuohy, M. G., & Koivula, A. (2010). Protein Engineering, Design & Selection, 23, 69–79.

    Article  CAS  Google Scholar 

  21. Heinzelman, P., Snow, C. D., Smith, M. A., Yu, X. L., Kannan, A., Boulware, K., Villalobos, A., Govindarajan, S., Minshull, J., & Arnold, F. H. (2009). The Journal of Biological Chemistry, 284, 26229–26233.

    Article  CAS  Google Scholar 

  22. Huang, Y., Krauss, G., Cottaz, S., Driguez, H., & Lipps, G. (2005). Biochemical Journal, 385, 581–588.

    Article  CAS  Google Scholar 

  23. Lee, Y. J., Kim, B. K., Lee, B. H., Jo, K. I., Lee, N. K., Chung, C. H., Lee, Y. C., & Lee, J. W. (2008). Bioresource Technology, 99, 378–386.

    Article  CAS  Google Scholar 

  24. Maki, M., Leung, K. T., & Qin, W. S. (2009). International Journal of Biological Sciences, 5, 500–516.

    Article  CAS  Google Scholar 

  25. Ni, J. F., Takehara, M., Miyazawa, M., & Watanabe, H. (2007). Protein Engineering, Design & Selection, 20, 535–542.

    Article  CAS  Google Scholar 

  26. Voutilainen, S. P., Puranen, T., Siika-aho, M., Lappalainen, A., Alapuranen, M., Kallio, J., Hooman, S., Viikri, L., Vehmaanpera, J., & Koivula, A. (2008). Biotechnolology and Bioengineerin, 101, 515–528.

    Article  CAS  Google Scholar 

  27. Camassola, M., De Bittencourt, L. R., Shenem, N. T., Andreaus, J., & Dillon, A. J. P. (2004). Biocatalysis and Biotransformation, 22, 391–396.

    Article  CAS  Google Scholar 

  28. Martins, L. F., Kolling, D., Camassola, M., Dillon, A. J. P., & Ramos, L. P. (2008). Bioresource Technology, 99, 1417–1424.

    Article  CAS  Google Scholar 

  29. Adsul, M. G., Bastawde, K. B., Varma, A. J., & Gokhale, D. V. (2007). Bioresource Technology, 98, 1467–1473.

    Article  CAS  Google Scholar 

  30. Vlasenko, E., Schülein, M., Cherry, J., & Xu, F. (2010). Bioresource Technology, 101, 2405–2411.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by the China Modern Agriculture Research System (CARS-42) and the Key Program of Transgenic Plant Breeding (2009ZX08003-020B) and the national science and technology support program (2011BADB02).

Author information

Authors and Affiliations

  1. Key Laboratory for Feed Biotechnology of the Ministry of Agriculture, Feed Research Institute, Chinese Academy of Agricultural Sciences, 12 Zhongguancun South Street, Beijing, 100081, People’s Republic of China

    Junqi Zhao, Pengjun Shi, Yingguo Bai, Huoqing Huang, Huiying Luo, Donghao Xu, Yaru Wang & Bin Yao

  2. College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Huitu Zhang

Authors
  1. Junqi Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Pengjun Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yingguo Bai
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Huoqing Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Huiying Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Huitu Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Donghao Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yaru Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Bin Yao
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Huitu Zhang or Bin Yao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhao, J., Shi, P., Bai, Y. et al. A Thermophilic Cellulase Complex from Phialophora sp. G5 Showing High Capacity in Cellulose Hydrolysis. Appl Biochem Biotechnol 166, 952–960 (2012). https://doi.org/10.1007/s12010-011-9483-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-011-9483-9

Keywords

Search

Navigation