Skip to main content
SpringerLink
Log in

Interspecies Interaction of Signal Peptide PapR Secreted by Bacillus cereus and Its Effect on Production of Antimicrobial Peptide

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

This study was carried out to investigate the interspecies interaction of PapR peptide secreted by Bacillus cereus on production of BSAP-254, an antimicrobial peptide produced by Bacillus subtilis SC-8 isolated from the Korean fermented soybean paste and exhibited narrow antagonistic activity against the B. cereus group, but not against other foodborne pathogens. PapR is a signal peptide that activates PlcR, which is a pleiotropic regulator controlling the expression of various virulence factors in B. cereus. When B. subtilis SC-8 was co-cultured with B. cereus, it completely inhibited the growth of B. cereus within 12 h, and the rate of BSAP-254 production was increased 34.2% at 12 h. Furthermore, 5 μM of synthetic PapR peptide added to the culture of B. subtilis SC-8 increased the rate of BSAP-254 production up to 59.7%. The growth of B. subtilis SC-8, however, was not significantly different with or without the addition of PapR. When B. cereus papR mutant was co-cultured with B. subtilis SC-8, the growth of the mutant was not inhibited and the rate of BSAP-254 production was decreased by 45%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Drobniewski, F. A. (1993). Clinical Microbiology Reviews, 6, 324–338.

    CAS  Google Scholar 

  2. Rasko, D. A., Altherr, M. R., Han, C. S., & Ravel, J. (2005). FEMS Microbiology Reviews, 29, 303–329.

    CAS  Google Scholar 

  3. Stenfors Arnesen, L. P., Fagerlund, A., & Granum, P. E. (2008). FEMS Microbiology Reviews, 32, 579–606.

    Article  CAS  Google Scholar 

  4. Bhunia, A. K. (2008). New York: Springer, pp. 135–142.

  5. Burgos, M. J. G., Kovács, Á. T., Mirończuk, A. M., Abriouel, H., Gálvez, A., & Kuipers, O. P. (2009). BMC Microbiology, 9, 227.

    Article  Google Scholar 

  6. Beecher, D. J., Pulido, J. S., Barney, N. P., & Wong, A. C. (1995). Infection and Immunity, 63, 632–639.

    CAS  Google Scholar 

  7. Miller, J. M., Hair, J. G., Hebert, M., Heber, L., Robert, F. J., & Weyant, R. S., Jr. (1997). Journal of Clinical Microbiology, 35, 504–507.

    CAS  Google Scholar 

  8. Arnaout, M., Tamburro, R., Bodner, S., Sandlund, J., Rivera, G., Pui, C., et al. (1999). Journal of Pediatric Hematology/Oncology, 21, 431–435.

    Article  CAS  Google Scholar 

  9. Hilliard, N. J., Schelonka, R. L., & Waites, K. B. (2003). Journal of Clinical Microbiology, 41, 3441–3444.

    Article  Google Scholar 

  10. Callegan, M. C., Kane, S. T., Cochran, D. C., Novosad, B., Gilmore, M. S., Gominet, M., et al. (2005). Investigative Ophthalmology & Visual Science, 46, 3233–3238.

    Article  Google Scholar 

  11. Baillie, L., & Read, T. D. (2001). Current Opinion in Microbiology, 24, 78–81.

    Article  Google Scholar 

  12. Mock, M., & Fouet, A. (2001). Annual Review of Microbiology, 55, 47–71.

    Article  Google Scholar 

  13. Jernigan, J. A., Stephens, D. S., Ashford, D. A., Omenaca, C., Topiel, M. S., Galbraith, M., et al. (2001). Emerging Infectious Diseases, 7, 933–944.

    Article  CAS  Google Scholar 

  14. Kortiranta, A., Lounatmaa, K., & Haapasalo, M. (2000). Microbes and Infection, 2, 189–198.

    Article  Google Scholar 

  15. Nicholson, W. L., Munakata, N., Horneck, G., Melosh, H. J., & Setlow, P. (2000). Microbiology and Molecular Biology Reviews, 64, 548–572.

    Article  CAS  Google Scholar 

  16. Fuqua, C., & Greenberg, E. P. (2002). Nature Reviews Molecular Cell Biology, 3, 685–695.

    Article  CAS  Google Scholar 

  17. Kleerebezem, M., Quadri, L. E., Kuipers, O. P., & de Vos, W. M. (1997). Molecular Microbiology, 24, 895–904.

    Article  CAS  Google Scholar 

  18. Økstad, O. A., Gominet, M., Purnelle, B., Rose, M., Lereclus, D., & Kolstø, A. B. (1999). Microbiology, 145, 3129–3138.

    Article  Google Scholar 

  19. Slamti, L., & Lereclus, D. (2002). EMBO Journal, 21, 4550–4559.

    Article  CAS  Google Scholar 

  20. Declerck, N., Bouillant, L., Chaix, D., Rugani, N., Slamti, L., Hoh, F., et al. (2007). Proceedings of the National Academy of Science of the United States of America, 104, 18490–18495.

    Article  CAS  Google Scholar 

  21. Bouillaut, L., Perchat, S., Arold, S., Zorrilla, S., Slamti, L., Henry, C., et al. (2008). Nucleic Acids Research, 36, 3791–3801.

    Article  CAS  Google Scholar 

  22. Gohar, M., Faegri, K., Perchat, S., Ravnum, S., Økstad, O. A., Gominet, M., et al. (2008). PLoS One, 3, e2793.

    Article  Google Scholar 

  23. Stein, T. (2005). Molecular Microbiology, 56, 845–857.

    Article  CAS  Google Scholar 

  24. Raaijmakers, J. M., de Bruijn, I., Nybroe, O., & Ongena, M. (2010). FEMS Microbiology Reviews, 34, 1037–1062.

    CAS  Google Scholar 

  25. Cladera-Olivera, F., Caron, G. R., & Brandelli, A. (2004). Letters in Applied Microbiology, 38, 251–256.

    Article  CAS  Google Scholar 

  26. Lee, N. K., Park, J. W., Cho, I. J., Kim, B. Y., Kwon, K. O., & Hahm, Y. T. (2008). Korean Journal Food Science and Technology, 40, 669–673.

    Google Scholar 

  27. Lee, N. K., Yeo, I. C., Park, J. W., Kang, B. S., & Hahm, Y. T. (2010). Journal of Bioscience and Bioengineering, 110, 298–303.

    Article  CAS  Google Scholar 

  28. Sivapathasekaran, C., Mukherjee, S., Samanta, R., & Sen, R. (2009). Analytical and Bioanalytical Chemistry, 395, 845–854.

    Article  CAS  Google Scholar 

  29. Pomerantsev, A. P., Pomerantseva, O. M., Camp, A. S., Radhika, R., Goldman, S., & Leppla, S. H. (2009). FEMS Immunology and Medical Microbiology, 55, 361–377.

    Article  CAS  Google Scholar 

  30. Maqueda, M., Sánchez-Hidalgo, M., Fernández, M., Montalbán-López, M., Valdivia, E., & Martinez-Bueno, M. (2008). FEMS Microbiology Reviews, 32, 2–22.

    Article  CAS  Google Scholar 

  31. Yeo, I. C., Lee, N. K., Cha, C. J., & Hahm, Y. T. (2011). Journal of Bioscience and Bioengineering, 112, 338–344.

    Article  CAS  Google Scholar 

  32. Lee, N. K., Yeo, I. C., Park, J. W., & Hahm, Y. T. (2011). Applied Biochemistry and Biotechnology, 165, 235–242.

    Article  CAS  Google Scholar 

  33. Straus, S. K., & Hancock, R. E. W. (2006). Biochimica et Biophysica Acta, 1758, 1215–1223.

    Article  CAS  Google Scholar 

  34. Røssland, E., Langsrud, T., Granum, P. E., & Sørhaug, T. (2005). International Journal of Food Microbiology, 98, 193–200.

    Article  Google Scholar 

  35. Atlas, R. M. (1986). & Bartha, R. Menlo Park: Benjamin-Cummings.

    Google Scholar 

  36. Camilli, A., & Bassler, B. L. (2006). Science, 311, 1113–1116.

    Article  CAS  Google Scholar 

  37. Romero, D., Traxler, M. F., López, D., & Kolter, R. (2011). Chemical Reviews, 111(9), 5492–5505.

    Article  CAS  Google Scholar 

  38. Sieprawska-Lupa, M., Mydel, P., Krawczyk, K., Wójcik, K., Puklo, M., Lupa, B., et al. (2004). Antimicrobial Agents and Chemotherapy, 48, 4673–4679.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

We thank Dr. Andrei P. Pomerantsev and Dr. Stephen H. Leppla (National Institutes of Health, Bethesda, MD, USA) for the gift of B. cereus papR mutant strain. This study was financially supported by the Basic Research Program (no. 2009–0073489) through the National Research Foundation of Korea (NRF) supported by the Ministry of Education, Science and Technology.

Author information

Authors and Affiliations

  1. Department of Biotechnology (BK21 Program), Chung-Ang University, Anseong, 456-756, Republic of Korea

    In-Cheol Yeo, Nam Keun Lee, Chang-Jun Cha & Young Tae Hahm

Authors
  1. In-Cheol Yeo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nam Keun Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chang-Jun Cha
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Young Tae Hahm
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Young Tae Hahm.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yeo, IC., Lee, N.K., Cha, CJ. et al. Interspecies Interaction of Signal Peptide PapR Secreted by Bacillus cereus and Its Effect on Production of Antimicrobial Peptide. Appl Biochem Biotechnol 166, 700–710 (2012). https://doi.org/10.1007/s12010-011-9459-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-011-9459-9

Keywords

Search

Navigation