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Additional Paper Waste in Pulping Sludge for Biohydrogen Production by Heat-Shocked Sludge

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Abstract

Dark anaerobic fermentation is an interesting alternative method for producing biohydrogen (H2) as a renewable fuel because of its low cost and various usable organic substrates. Pulping sludge from wastewater treatment containing plentiful cellulosic substrate could be feasibly utilized for H2 production by dark fermentation. The objective of this study was to investigate the optimal proportion of pulping sludge to paper waste, the optimal initial pH, and the optimal ratio of carbon and nitrogen (C/N) for H2 production by anaerobic seed sludge pretreated with heat. The pulping sludge was pretreated with NaOH solution at high temperature and further hydrolyzed with crude cellulase. Pretreatment of the pulping sludge with 3% NaOH solution under autoclave at 121 °C for 2 h, hydrolysis with 5 FPU crude cellulase at 50 °C, and pH 4.8 for 24 h provided the highest reducing sugar production yield (229.68 ± 2.09 mg/gTVS). An initial pH of 6 and a C/N ratio of 40 were optimal conditions for H2 production. Moreover, the supplement of paper waste in the pulping sludge enhanced the cumulative H2 production yield. The continuous hydrogen production was further conducted in a glass reactor with nylon pieces as supporting media and the maximum hydrogen production yield was 151.70 ml/gTVS.

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Acknowledgments

The authors gratefully acknowledge financial support by Thailand Research Fund through Grant Number MRG5080301.

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Authors and Affiliations

  1. Environmental Technology Unit, Department Environmental Science, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

    Prapaipid Chairattanamanokorn, Supachok Tapananont, Siriporn Detjaroen, Juthatip Sangkhatim & Patana Anurakpongsatorn

  2. Research Group for Development of Microbial Hydrogen Production Process from Biomass, Bangkok, Thailand

    Prapaipid Chairattanamanokorn

  3. Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

    Pramote Sirirote

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  1. Prapaipid Chairattanamanokorn
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Correspondence to Prapaipid Chairattanamanokorn.

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Chairattanamanokorn, P., Tapananont, S., Detjaroen, S. et al. Additional Paper Waste in Pulping Sludge for Biohydrogen Production by Heat-Shocked Sludge. Appl Biochem Biotechnol 166, 389–401 (2012). https://doi.org/10.1007/s12010-011-9434-5

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