Skip to main content
SpringerLink
Log in

Xylanase and β-Xylosidase Production by Aspergillus ochraceus: New Perspectives for the Application of Wheat Straw Autohydrolysis Liquor

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

The xylanase biosynthesis is induced by its substrate—xylan. The high xylan content in some wastes such as wheat residues (wheat bran and wheat straw) makes them accessible and cheap sources of inducers to be mainly applied in great volumes of fermentation, such as those of industrial bioreactors. Thus, in this work, the main proposal was incorporated in the nutrient medium wheat straw particles decomposed to soluble compounds (liquor) through treatment of lignocellulosic materials in autohydrolysis process, as a strategy to increase and undervalue xylanase production by Aspergillus ochraceus. The wheat straw autohydrolysis liquor produced in several conditions was used as a sole carbon source or with wheat bran. The best conditions for xylanase and β-xylosidase production were observed when A. ochraceus was cultivated with 1% wheat bran added of 10% wheat straw liquor (produced after 15 min of hydrothermal treatment) as carbon source. This substrate was more favorable when compared with xylan, wheat bran, and wheat straw autohydrolysis liquor used separately. The application of this substrate mixture in a stirred tank bioreactor indicated the possibility of scaling up the process to commercial production.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Myerly, R. C., Nicholson, M. D., Katzen, R., & Taylor, J. M. (1981). Chemtech, 11, 186–192.

    CAS  Google Scholar 

  2. Moure, A., Gullón, P., Domínguez, H., & Parajó, J. C. (2006). Process Biochemistry, 41, 1913–1923.

    Article  CAS  Google Scholar 

  3. Garrote, G., Domínguez, H., & Parajó, J. C. (2002). Journal of Food Engineering, 52, 211–218.

    Article  Google Scholar 

  4. Garrote, G., Falqué, E., Domínguez, H., & Parajó, J. C. (2007). Bioresource Technology, 98, 1951–1957.

    Article  CAS  Google Scholar 

  5. Ebringerova, A., & Heinze, T. (2000). Macromolecular Rapid Communications, 21, 542–556.

    Article  CAS  Google Scholar 

  6. Abdel-Sater, M. A., & El-Said, A. H. M. (2001). International Biodeterioration and Biodegradation, 47, 15–21.

    Article  CAS  Google Scholar 

  7. Polizeli, M. L. T. M., Rizzatti, A. C. S., Monti, R., Terenzi, H. F., Jorge, J. A., & Amorim, D. S. (2005). Applied Microbiology and Biotechnology, 67, 577–591.

    Article  CAS  Google Scholar 

  8. Liu, C., Sun, Z.-T., Du, J.-H., & Wang, J. (2008). Journal of Industrial Microbiology and Biotechnology, 35(7), 703–711.

    Article  CAS  Google Scholar 

  9. Polizeli, M. L. T. M. (2009). Properties and commercial applications of xylanases from fungi. In M. Rai (Ed.), Advances in fungal biotechnology (Vol. 1, pp. 82–108). New Delhi: IK International Publisher.

    Google Scholar 

  10. Katapodis, P., Christakopoulou, V., Kekos, D., & Christakopoulou, P. (2007). Biochemical Engineering Journal, 35, 136–141.

    Article  CAS  Google Scholar 

  11. Subramanyan, S., & Prema, P. (2002). Critical Reviews in Biotechnology, 22, 33–64.

    Article  Google Scholar 

  12. Reddy, V., Reddy, P., Pillay, B., & Singh, S. (2002). Process Biochemistry, 37, 1221–1228.

    Article  CAS  Google Scholar 

  13. Adams, P. R. (1990). Mycopathologia, 112, 35–37.

    Article  Google Scholar 

  14. Miller, G. H. (1959). Analytical Chemistry, 31, 426–429.

    Article  CAS  Google Scholar 

  15. Kersters-Hilderson, H., Claeyssens, M., Doorslaer, E. V., Saman, E., & Bruyne, C. K. (1982). Methods in Enzymology, 83, 631–639.

    Article  CAS  Google Scholar 

  16. Techapun, C., Poosaran, N., Watanabe, M., & Sasaki, K. (2003). Process Biochemistry, 38, 1327–1340.

    Article  CAS  Google Scholar 

  17. Michelin, M., Peixoto-Nogueira, S. C., Betini, J. H. A., Silva, T. M., Jorge, J. A., Terenzi, H. F., et al. (2010). Bioprocess Biosyst Engineer, 33, 813–821.

    Article  CAS  Google Scholar 

  18. Michelin, M., Polizeli, M. L. T. M., Silva, D. P., Ruzene, D. S., Vicente, A. A., Jorge, J. A., Terenzi, H. F., & Teixeira, J. A. (2011). Journal of Industrial Microbiology and Biotechnology. doi:10.1007/s10295-011-0987-7.

  19. Parajó, J. C., Garrote, G., Cruz, J. M., & Dominguez, H. (2004). Trends in Food Science and Technology, 15, 115–120.

    Article  Google Scholar 

  20. Sanchez, Z. B., & Bautista, J. (1988). Enzyme and Microbial Technology, 10, 315–318.

    Article  CAS  Google Scholar 

  21. Felipe, M. G. A., Mancilha, I. M., Vitolo, M., Roberto, I. C., Silva, S. S., & Rosa, S. A. M. (1993). Arquivos de Biologia e Tecnologia, 36, 103–114.

    CAS  Google Scholar 

  22. Olsson, L., & Hahn-Hagerdal, B. (1996). Enzyme and Microbial Technology, 18, 312–331.

    Article  CAS  Google Scholar 

  23. Kalathenos, P., Baranyi, J., Sutherland, J. P., & Roberts, T. A. (1995). International Journal of Food Microbiology, 25, 63–74.

    Article  CAS  Google Scholar 

  24. Moyo, S., Gashe, B. A., Collison, E. K., & Mpuchane, S. (2003). International Journal of Food Microbiology, 85, 87–100.

    Article  CAS  Google Scholar 

  25. Fang, H. Y., Chang, S. M., Hsieh, M. C., & Fang, T. J. (2007). Journal of Molecular Catalysis B: Enzymatic, 49, 36–42.

    Article  CAS  Google Scholar 

  26. Coelho, G. D., & Carmona, E. C. (2003). Journal of Basic Microbiology, 43(4), 269–277.

    Article  CAS  Google Scholar 

  27. Haltrich, D., Nidetsky, B., Kulbe, K. D., Steiner, W., & Zupancic, S. (1996). Bioresource Technology, 58, 137–161.

    Article  CAS  Google Scholar 

  28. Beg, Q. K., Bhushan, B., Kapoor, M., & Hoondal, G. S. (2000). Enzyme and Microbial Technology, 27, 459–466.

    Article  CAS  Google Scholar 

  29. Siedenberg, D., Gerlach, S. R., Czwalinna, A., Schugerl, K., Giuseppin, M. L. F., & Hunik, J. (1997). Journal of Biotechnology, 56, 205–216.

    Article  CAS  Google Scholar 

  30. Hoq, M. M., Hempel, C., & Deckwer, W.-D. (1994). Journal of Biotechnology, 37, 49–59.

    Article  CAS  Google Scholar 

  31. Palma, M. B., Milagres, A. M. F., Prata, A. M. R., & de Mancilha, I. M. (1996). Process Biochemistry, 31, 141–145.

    Article  CAS  Google Scholar 

  32. Singh, S., du Preez, J. C., Pillay, B., & Prior, B. A. (2000). Applied Microbiology and Biotechnology, 54, 698–704.

    Article  CAS  Google Scholar 

  33. Silva, D. P., Pessoa, A., Jr., Roberto, I. C., & Vitolo, M. (2001). Applied Biochemistry and Biotechnology, 91–93, 605–613.

    Article  Google Scholar 

  34. Techapun, C., Poosaran, N., Watanabe, M., & Sasaki, K. (2003). Journal of Bioscience and Bioengineering, 95, 298–301.

    CAS  Google Scholar 

  35. Chipeta, Z. A., du Preez, J. C., & Christopher, L. (2008). Journal of Industrial Microbiology and Biotechnology, 35(6), 587–594.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP/Brazil), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/Brazil), National System for Research on Biodiversity (SISBIOTA-Brazil, CNPq 563260/2010-6/FAPESP no. 2010/52322-3), and Fundação para a Ciência e a Tecnologia (FCT/Portugal).

Author information

Authors and Affiliations

  1. IBB–Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057, Braga, Portugal

    Michele Michelin, Denise S. Ruzene, Daniel P. Silva, António A. Vicente & José A. Teixeira

  2. Department of Biology, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, 14040-901, Ribeirão Preto, São Paulo, Brazil

    Michele Michelin, Maria de Lourdes T. M. Polizeli, João A. Jorge & Héctor F. Terenzi

  3. Institute of Technology and Research, University Tiradentes, Campus Farolândia, 49032-490, Aracaju, Sergipe, Brazil

    Denise S. Ruzene & Daniel P. Silva

Authors
  1. Michele Michelin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maria de Lourdes T. M. Polizeli
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Denise S. Ruzene
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Daniel P. Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. António A. Vicente
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. João A. Jorge
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Héctor F. Terenzi
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. José A. Teixeira
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Daniel P. Silva.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Michelin, M., Polizeli, M.L.T.M., Ruzene, D.S. et al. Xylanase and β-Xylosidase Production by Aspergillus ochraceus: New Perspectives for the Application of Wheat Straw Autohydrolysis Liquor. Appl Biochem Biotechnol 166, 336–347 (2012). https://doi.org/10.1007/s12010-011-9428-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-011-9428-3

Keywords

Search

Navigation