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Chemiluminescent Detection of Carbohydrates in the Tumoral Breast Diseases

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Abstract

Nowadays, there is an increase of investigations into the fibroadenoma, mainly because some studies have shown that the occurrence of fibroadenoma is linked to an increased risk of developing breast carcinoma. Currently, the chemiluminescence biomarkers are applied for validation methods and screening. Here, a lectin chemiluminescence is proposed as new histochemistry method to identify carbohydrates in mammary tumoral tissues. The lectins concanavalin A (Con A) and peanut agglutinin (PNA) conjugated to acridinium ester were used to characterize the glycocode of breast tissues: normal, fibroadenoma, and invasive duct carcinoma (IDC). The lectin chemiluminescence expressed in relative light units (RLU) was higher in fibroadenoma and IDC than in normal tissue for both lectins tested. The relationship RLU emission versus tissue area described a linear and hyperbolic curve for IDC and fibroadenoma, respectively, using Con A whereas hyperbolic curves for both transformed tissues using PNA. RLU was abolished by inhibiting the interaction between tissues and lectins using their specific carbohydrates: methyl-α-d-mannoside (Con A) and galactose (PNA). The intrinsic fluorescence emission did not change with combination of the lectins (Con A/PNA) to the acridinium ester for hydrophobic residues. These results represent the lectin chemiluminescence as an alternative of histochemistry method for tumoral diagnosis in the breast.

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Acknowledgments

Authors thank to CNPq and FACEPE for financial support as well as to Ian P. G. Amaral for the statistical analysis.

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Authors and Affiliations

  1. Laboratório de Imunopatologia Keizo Asami, LIKA/UFPE, Recife, Brazil

    Vanessa Passos Brustein, Carmelita Lima Bezerra Cavalcanti, Mario Ribeiro de Melo-Junior, Eduardo Isidoro Carneiro Beltrão & Luiz Bezerra Carvalho Jr.

  2. Departamento de Química Fundamental, DQF/UFPE, Recife, Brazil

    Vanessa Passos Brustein

  3. Departamento de Patologia, CCS/UFPE, Recife, Brazil

    Mario Ribeiro de Melo-Junior

  4. Departamento de Bioquímica, CCB/UFPE, Recife, Brazil

    Maria Tereza Santos Correia, Eduardo Isidoro Carneiro Beltrão & Luiz Bezerra Carvalho Jr.

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  1. Vanessa Passos Brustein
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  2. Carmelita Lima Bezerra Cavalcanti
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  3. Mario Ribeiro de Melo-Junior
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  4. Maria Tereza Santos Correia
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  5. Eduardo Isidoro Carneiro Beltrão
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  6. Luiz Bezerra Carvalho Jr.
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Correspondence to Vanessa Passos Brustein.

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Brustein, V.P., Cavalcanti, C.L.B., de Melo-Junior, M.R. et al. Chemiluminescent Detection of Carbohydrates in the Tumoral Breast Diseases. Appl Biochem Biotechnol 166, 268–275 (2012). https://doi.org/10.1007/s12010-011-9422-9

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  • DOI: https://doi.org/10.1007/s12010-011-9422-9

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