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New Feruloyl Esterases to Access Phenolic Acids from Grass Biomass

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Abstract

In the Sorangium cellulosum strain So ce56 genome, two putative esterase-encoding genes (loci sce1896 and sce8927) were cloned, expressed in Escherichia coli, and the resulting enzymes (designated ScFAE1 and ScFAE2) were used to assess the possible release of ferulic acid (FA) from triticale and wheat brans, and an aqueous fraction of steam-exploded wheat straw. The two polypeptides, sharing only 30% sequence identity, exhibit a typical catalytic Ser-Asp-His triad, a characteristic of α/β-hydrolase fold proteins. Both ScFAE1 (35 kDa) and ScFAE2 (34 kDa) were purified to apparent homogeneity and comparison of their kinetic parameters indicated an apparent higher affinity of ScFAE2 than ScFAE1 towards the various feruloyl substrates. This property was reflected by the observation that ScFAE2 was capable of yielding up to 85% of FA from destarched triticale bran. In the steam-exploded wheat sample, more than 85% yield of FA or p-coumaric acid was also effected by ScFAE2 without the decomposition of valuable chemical such as furfural. The two cloned FAEs represent the first of myxobacterial origin to be characterized and they are classified as new members of the type D family of FAEs.

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Acknowledgments

This work was supported by funding through the Canadian Triticale Biorefinery Initiative (CTBI) of the Agricultural Bioproducts Innovation Program of Agriculture and Agri-Food Canada (AAFC) and the National Bioproducts Program of NRCC-AFCC-NRCan. We thank A. Corriveau and C. Beaulieu for their expert help in various analytical analyses. Stephen Allen of Bio Vision is thanked for providing the steam explosion aqueous sample and brief information about the process.

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  1. Biotechnology Research Institute, National Research Council Canada, 6100 Royalmount Avenue, Montreal, QC, H4P 2R2, Canada

    Meiqun Wu, Kofi Abokitse, Stephan Grosse, Hannes Leisch & Peter C. K. Lau

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  1. Meiqun Wu
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  2. Kofi Abokitse
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Correspondence to Peter C. K. Lau.

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Wu, M., Abokitse, K., Grosse, S. et al. New Feruloyl Esterases to Access Phenolic Acids from Grass Biomass. Appl Biochem Biotechnol 168, 129–143 (2012). https://doi.org/10.1007/s12010-011-9359-z

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