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Fermentative Hydrogen Production from Soybean Protein Processing Wastewater in an Anaerobic Baffled Reactor (ABR) Using Anaerobic Mixed Consortia

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Abstract

Fermentative H2 production from soybean protein processing wastewater (SPPW) was investigated in a four-compartment anaerobic baffled reactor (ABR) using anaerobic mixed cultures under continuous flow condition in the present study. After being inoculated with aerobic activated sludge and operated at the inoculants of 5.98 gVSS L−1, COD of 5000 mg L−1, HRT of 16 h and temperature of (35 ± 1) °C for 22 days, the ABR achieved stable ethanol-type fermentation. The specific hydrogen production rate of anaerobic activated sludge was 165 LH2 kg MLVSS−1 day−1, the substrate conversion rate was 600.83 LH2 kg COD−1and the COD removal efficiency was 44.73% at the stable operation status. The ABR system exhibited a better stability and higher hydrogen yields than continuous stirring tank reactor under the same operational condition. The experimental data documented the feasibility of substrate degradation along with molecular H2 generation utilizing SPPW as primary carbon source in the ABR system.

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Acknowledgments

The authors would like to thank the National Natural Science Foundation of China (Contract No. 50808152), Provincial Science Foundation of Fujian (Grant No. 2010 J01314) and the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KZCX2-EW-402-02) for their supports for this study.

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Authors and Affiliations

  1. Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China

    Ge-fu Zhu & Chao-xiang Liu

  2. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, China

    Jian-zheng Li

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  1. Ge-fu Zhu
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  2. Jian-zheng Li
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Correspondence to Ge-fu Zhu.

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Zhu, Gf., Li, Jz. & Liu, Cx. Fermentative Hydrogen Production from Soybean Protein Processing Wastewater in an Anaerobic Baffled Reactor (ABR) Using Anaerobic Mixed Consortia. Appl Biochem Biotechnol 168, 91–105 (2012). https://doi.org/10.1007/s12010-011-9357-1

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  • DOI: https://doi.org/10.1007/s12010-011-9357-1

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