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Investigation of the Effective Action Distance Between Hematopoietic Stem/Progenitor Cells and Human Adipose-Derived Stem Cells During Their In Vitro Co-culture

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Abstract

The in vitro suitable action distance between umbilical cord blood-derived hematopoietic stem/progenitor cells and its feeder cell, human adipose-derived stem cells, during their co-culture, was investigated through a novel transwell co-culture protocol, in which the distance between the two culture chambers where each cell type is growing can be adjusted from 10 to 450 μm. The total cell number was determined with a hemacytometer, and the cell morphology was observed under an inverted microscope each day. After 7 days of co-culture, the fold-expansion, surface antigen expression of CD34+ and CFU-GM assay of the hematopoietic mononuclear cells (MNCs) were analyzed. The results showed that there was an optimal communication distance at around 350 μm between both types of stem cells during their in vitro co-culture. By using this distance, the UCB-MNCs and CD34+ cells were expanded by 15.1 ± 0.2 and 5.0 ± 0.1-fold, respectively. It can therefore be concluded that the optimal action distance between stem cells and their supportive cells, when cultured together for 7 days, is of around 350 μm.

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Acknowledgments

This work was supported by The National Natural Sciences Foundation of China (30670525, 30700181), the Fundamental Research Funds for the Central Universities (DUT11SM09), and Doctoral Fund of Ministry of Education of China (20070141055).

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Authors and Affiliations

  1. Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian, 116024, China

    Kedong Song, Hai Wang, Mo Qiao, Shuang Wu & Tianqing Liu

  2. Department of Orthopaedics, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China

    Hong Wang

  3. Department of Oncology, First Affiliated Hospital of Dalian Medical University, Dalian, 116011, China

    Ling Wang

Authors
  1. Kedong Song
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  2. Hai Wang
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  3. Hong Wang
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  4. Ling Wang
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  5. Mo Qiao
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  6. Shuang Wu
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  7. Tianqing Liu
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Corresponding author

Correspondence to Tianqing Liu.

Additional information

Kedong Song, Hai Wang, Hong Wang, and Ling Wang contributed equally to this work.

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Song, K., Wang, H., Wang, H. et al. Investigation of the Effective Action Distance Between Hematopoietic Stem/Progenitor Cells and Human Adipose-Derived Stem Cells During Their In Vitro Co-culture. Appl Biochem Biotechnol 165, 776–784 (2011). https://doi.org/10.1007/s12010-011-9295-y

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  • DOI: https://doi.org/10.1007/s12010-011-9295-y

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