Abstract
Pancreatic cancer is a malignant tumor with the worst prognosis among all cancers. At the time of diagnosis, surgical cure is no longer a feasible option for most patients, thus early detection of pancreatic cancer is crucial for its treatment. Metabolomics is a powerful new analytical approach to detect the metabolome of cells, tissue, or biofluids. Here, we report the application of 1H nuclear magnetic resonance (NMR) combined with principal components analysis to discriminate pancreatic cancer patients from healthy controls based on metabolomic profiling of the serum. The metabolic analysis revealed significant lower of 3-hydroxybutyrate, 3-hydroxyisovalerate, lactate, and trimethylamine-N-oxide as well as significant higher level of isoleucine, triglyceride, leucine, and creatinine in the serum from pancreatic cancer patients compared to that of healthy controls. Our data demonstrate that the subtle differences in metabolite profiles in serum of pancreatic cancer patients and that of healthy subjects as a result of physiological and pathological variations could be identified by NMR-based metabolomics and exploited as metabolic markers for the early detection of pancreatic cancer.
Similar content being viewed by others
References
Jemal, A., Siegel, R., Ward, E., Hao, Y., Xu, J., & Murray, T. (2008). Thun MJ. CA: A Cancer Journal for Clinicians, 58, 71–96.
Sener, S. F., Fremgen, A., Menck, H. R., & Winchester, D. P. (1999). Journal of the American College of Surgeons, 189, 1–7.
Bollard, M. E., Stanley, E. G., Lindon, J. C., Nicholson, J. K., & Holmes, E. (2005). NMR in Biomedicine, 18, 143–162.
Holmes, E., Foxall, P. J. D., Spraul, M., Farrant, R. D., Nicholson, J. K., & Lindon, J. C. (1997). Journal of Pharmaceutical and Biomedical Analysis, 15, 1647–1659.
Baran, E. T., Ozer, N., & Hasirci, V. (2002). Journal of Microencapsulation, 19(3), 363–376.
Kim, J. M., Song, J. S., Cho, H. H., Shin, K. K., Bae, Y. C., Lee, B. J., et al. (2011). Molecular and Cellular Biochemistry, 346(1–2), 125–136.
Kim, K. B., Yang, J. Y., Kwack, S. J., Park, K. L., Kim, H. S., Ryu, D. H., et al. (2010). Journal of Toxicology and Environmental Health. Part A, 73(21–22), 1420–1430.
DeWaele, B., Vanmierlo, B., Van Nieuwenhove, Y., & Delvaux, G. (2006). Pancreas, 32, 343–345.
Martinez, J., Johnson, C. D., Sanchez-Paya, J., de Madaria, E., Robles-Diaz, G., & Perez-Mateo, M. (2006). Pancreatology, 6, 206–209.
Papachristou, G. I., Papachristou, D. J., Avula, H., Slivka, A., & Whitcomb, D. C. (2006). Pancreatology, 6, 279–285.
Suazo-Barahona, J., Carmona-Sanchez, R., Robles-Diaz, G., Milke-Garcia, P., Vargas-Vorackova, F., Uscanga-Dominguez, L., et al. (1998). The American Journal of Gastroenterology, 93, 1324–1328.
Calle, E. E., Rodriguez, C., Walker-Thurmond, K., & Thun, M. J. (2003). The New England Journal of Medicine, 348, 1625–1638.
Giovannucci, E., & Michaud, D. (2007). Gastroenterology, 132, 2208–2225.
Michaud, D. S., Giovannucci, E., Willet, W. C., Colditz, G. A., Stampfer, M. J., & Fuchs, C. S. (2001). JAMA., 286, 921–929.
Patel, A. V., Rodriguez, C., Bernstein, L., Chao, A., Thun, M. J., & Calle, E. E. (2005). Cancer Epidemiology, Biomarkers and Prevention, 14, 459–466.
Muddana, V., Whitcomb, D. C., Khalid, A., Slivka, A., & Papachristou, G. I. (2009). The American Journal of Gastroenterology, 104(1), 164–170.
Hiraoka, N., Ino, Y., Sekine, S., Tsuda, H., Shimada, K., Kosuge, T., et al. (2010). British Journal of Cancer, 103(7), 1057–1065.
Brizel, D. M., Schroeder, T., Scher, R. L., Walenta, S., Clough, R. W., & Dewhirst, M. W. (2001). International Journal of Radiation Oncology, Biology, Physics, 51, 349–353.
Schwickert, G., Walenta, S., Sundfor, K., Rofstad, E. K., & Mueller-Klieser, W. (1995). Cancer Research, 55, 4757–4759.
Walenta, S., Chau, T. V., Schroeder, T., Lehr, H. A., Kunz-Schughart, L. A., Fuerst, A., et al. (2003). Journal of Cancer Research and Clinical Oncology, 129, 321–326.
Author information
Authors and Affiliations
Corresponding authors
Rights and permissions
About this article
Cite this article
OuYang, D., Xu, J., Huang, H. et al. Metabolomic Profiling of Serum from Human Pancreatic Cancer Patients Using 1H NMR Spectroscopy and Principal Component Analysis. Appl Biochem Biotechnol 165, 148–154 (2011). https://doi.org/10.1007/s12010-011-9240-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-011-9240-0